孕鼠免疫激活对其子鼠调节性CD4^+CD25^+T细胞分化及功能影响的初步研究  

The Preliminary Functional Study of CD4^+CD25^+T Cells from Adult LPS Offspring Born to Dam with LPS Exposure on Gestational Day 12.5

作　　者：栾荣[1] 成浩[1] 赵强[1] 吴震州[1] 赵立青[1] 

机构地区：[1]南开大学生命科学学院,天津300071

出　　处：《南开大学学报（自然科学版）》2015年第6期99-104,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金(31170858;30007101);天津市应用基础及前沿技术研究计划(12JCYBJC15700)

摘　　要：CD4^+CD25^+T细胞是一种重要的调节性T细胞,在机体免疫反应中发挥着重要作用,探明孕鼠感染细菌对其子鼠调节性T细胞分化和功能的影响具有一定的生物学意义。用LPS介导的孕鼠免疫激活模型来模拟孕妇细菌感染.发现50μg/kg LPS诱导妊娠12.5 d的C57BL/6孕鼠产生大量的炎症细胞因子IL-6和TNF-α.孕鼠免疫激活导致成年LPS子鼠脾脏CD4^+CD25^+调节性T细胞增多。在实验性自身免疫性脑脊髓炎动物模型中成年LPS子鼠出现免疫耐受.免疫组LPS子鼠的外周淋巴结中CD4^+CD25^+调节性T细胞增多。该工作为后继深入探讨孕鼠免疫激活是如何调控成年LPS子鼠CD4^+CD25^+调节性T细胞的表达和功能奠定了一定的基础.CD^＋CD25^＋ T cells play important regulatory roles during immune response. To understand the effect of LPS exposure on gestational day 12.5 on frequency and functional changes of CD4^＋CD25^＋ T cells from adult LPS offspring, we established C57BL/6J mouse model of maternal immune activation by intraperitoneal injection with 50 μg/kg I.PS on gestational day 12.5. There was a large amount serum IL 6 and TNF-α two hours after gestational exposure with LPS. In comparison with adult PBS offspring, there was significantly elevated frequency of CD4^＋CD25^＋T cells from adult LPS offspring and adult LPS offspring showed resistance to experimental allergic encephalomyelitis induction. Besides, increased frequency of CD4^＋CD25v＋ T cells was found in adult I.PS offspring by comparing with PBS offspring 22 days post MOG （33-55） immunization. Our results provided the foundation for further research of how maternal immune activation modulate the expression and function of CD4＋CD25^＋ T cells of adult LPS offspring.

关 键 词：孕鼠免疫激活 lipopolysaccharide CD4^+CD25^+T细胞 实验性自身免疫性脑脊髓炎 

分 类 号：Q813[生物学—生物工程]