禽网状内皮组织增生病病毒感染对SPF鸡免疫器官和疫苗免疫效果的影响  

The Influence of Reticuloendotheliosis Virus Infection on Immune Organs and Immune Efficacy in SPF Chickens

作　　者：李凯[1] 高立[1] 祁小乐[1] 高宏雷[1] 高玉龙[1] 王永强[1] 王笑梅[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,禽免疫抑制病研究团队,哈尔滨150001

出　　处：《畜牧兽医学报》2016年第2期340-345,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家国际科技合作专项项目(2011DFA32210);农业科技成果转化资金项目(2013GB23260577);国家现代农业产业技术体系(nycytx-42-G3-01)

摘　　要：禽网状内皮组织增生病(RE)是禽类的一种重要免疫抑制性疾病,目前对其造成免疫抑制的机制尚不十分清楚。本研究对实验室分离的一株REV流行毒株(HLJR0901株)感染1日龄SPF鸡后对感染鸡免疫器官和免疫功能造成的影响进行研究。结果表明,1日龄SPF鸡感染REV后出现生长迟缓,法氏囊、胸腺萎缩,脾肿大的症状。感染鸡出现法氏囊滤泡淋巴细胞大量坏死减少,间质结缔组织增生;胸腺出血,胸腺小体增多;脾小结明显萎缩,淋巴细胞减少等病理变化。荧光定量PCR检测发现,病毒在感染鸡法氏囊、胸腺和脾内均有分布,且持续存在。SPF鸡感染REV后出现严重的免疫抑制,使AIV灭活疫苗和NDV弱毒疫苗的免疫效果显著下降。本研究为阐明REV的免疫抑制机制提供了重要的实验数据。Reticuloendotheliosis（RE）is an important avian immunosuppressive disease with the mechanism of the immunosuppression barely known.In this study,the influence of a REV strain,HLJR0901 isolated in our lab,infection on the immune organs and immunology competence in SFP chickens was examined.Decline in body weight,atrophy in bursa of Fabricius and thymus,and tumefaction in spleen were detected after REV infection.Histopathology study observed several pathological changes in the main immune organs,including the necrosis and number decline of the immune cells and the hyperplasia of the connective tissues in the bursa of Fabricius,the haemorrhagia and increasing of the thymic corpuscle in the thymus,and atrophy of the splenic follicle in the spleen.High proviral loads were detected in the bursa of Fabricius,thymus and spleen with a long persistence.Additionally,the antibody responses to AIV and NDV vaccines were significantly inhibited by REV early infection compared with the control chickens.This study provided important data for illuminating the mechanism of the REV-induced immunosuppression.

关 键 词：禽网状内皮组织增生病 致病性 免疫器官 免疫抑制 

分 类 号：S852.659.3[农业科学—基础兽医学]