黄芩苷对结肠癌细胞迁移与侵袭能力的抑制作用及其机制研究  

The inhibition and mechanism of baicalin on migration and invasion of colon cancer cells

作　　者：吴汪泽[1] 王卉[1] 卢忠心[1] 

机构地区：[1]武汉市中心医院检验科

出　　处：《现代预防医学》2016年第4期708-710,726,共4页Modern Preventive Medicine

摘　　要：目的研究黄芩苷对人结肠癌SW480细胞增殖、迁移和侵袭的影响,并初步探讨其作用机制。方法采用MTT法、细胞划痕实验和Transwell实验检测黄芩苷对SW480细胞增殖、迁移和侵袭能力的作用,Western blot方法检测黄芩苷作用后SW480细胞中MMP-9和COX-2的蛋白表达水平。结果黄芩苷以时间和浓度依赖方式抑制SW480细胞的增殖;黄芩苷能明显抑制SW480细胞的迁移和侵袭能力(P=0.0012,P=0.0017);并且黄芩苷能够下调SW480细胞中MMP-9和COX-2蛋白的表达(P=0.0013,P=0.0036)。结论黄芩苷可能通过下调MMP-9和COX-2蛋白的表达,从而发挥抑制SW480细胞增殖、迁移和侵袭的作用。Objective The study was conducted to assess the influence of baicalin on proliferation, migration, and invasion of colon cancer SW480 cells and to preliminarily explore its mechanism of action. Methods The effects of baicalin on proliferation, migration, and invasion of SW480 cells were examined by MTT assay, scratch assay, and Transwell assay. Expressions of MMP-9 and COX-2 in baicalin-treated SW480 cells were determined by Western blot. Results Baicalin exhibited a time and density-dependent inhibition on the proliferation of SW480 cells. Baicalin was also shown to significantly suppress the migration and invasion of SW480 cells （P=0.0012, P=0.0017） and reduce the expressions of MMP-9 and COX-2 in SW480 cells （P=0.0013, P=0.0036）. Conclusion Baicalin inhibits the proliferation, migration and invasion of SW480 cells possibly by down-regulating the expression of MMP-9 and COX-2 proteins.

关 键 词：黄芩苷 结肠癌 增殖 迁移 侵袭 

分 类 号：R319[医药卫生—基础医学]