应用双向电泳分析结核性脑膜炎与隐球菌性脑膜炎脑脊液的蛋白差异  

The analysis of protein differences in cerebrospinal fluid of tuberculous meningitis and cryptococcus meningitis by two-dimensional electrophoresis

作　　者：黄绍梅[1] 杨瑜[1] 邱薇[1] 吴睿彦[1] 谢贝[1] 刘志辉[1] 陈志成[1] 

机构地区：[1]广州市胸科医院,510095

出　　处：《国际医药卫生导报》2016年第3期300-302,共3页International Medicine and Health Guidance News

基　　金：广东省科技计划项目（2012A030400034）;广州市科技计划项目（2014Y2-00117）

摘　　要：目的比较分析结核性脑膜炎（TBM）与隐球菌性脑膜炎脑脊液的差异蛋白质，筛选二者的鉴别诊断标志物。方法采用双向凝胶电泳技术对4例TBM和4例隐球菌性脑膜炎脑脊液蛋白进行电泳分离，以PDQuest8．0软件根据蛋白的等电点PI、分子量、匹配率、灰度值等进行差异蛋白描述性分析。结果结核性、隐球菌性两组脑脊液分别检测到蛋白点数为111和114，蛋白点分子量均分布为10～130kDa，等电点PI为4．4～6．7间。两组脑脊液蛋白图谱匹配率为95％，产生24个差异蛋白点，其中3个蛋白点只在TBM中表达，2个蛋白点只在隐球菌性脑膜炎中表达，19个匹配蛋白点存在2倍差异表达。结论双向电泳技术可得到分辨率和重复率较高的结核性与隐球菌性脑膜炎脑脊液蛋白质凝胶图谱，二者存在差异蛋白点。Objective To screen the diagnostic markers between tuberculous meningitis and cryptococcus meningitis by analyzing the protein differences in cerebrospinal fluid （CSF）. Methods The proteins in CSF of 4 cases of tuberculous meningitis and 4 cases of cryptococcal meningitis were separated by two-dimensional gel electrophoresis （2-DE）. The differential proteins expression was descriptively analyzed according to the protein isoelectric point PI, molecular weight, matching rate, gray value by PDQuestS.0 software. Results 111 and 114 protein spots were captured from CSF of tuberculose meningitis and cryptococcus meningitis. The molecular weight of proteins ranged from 10 to 130 kDa. The isoelectric point （PI） was from 4.4 to 6.7. The matching rate of two groups was 95%. There were 24 differential proteins between two groups, of which 3 proteins were expressed only in tuberculous meningitis and 2 proteins expressed only in cryptococcal meningitis. 19 matching protein point were two-fold differential expression. Conclusion 2-DE profiles with high resolution and reproducibility of CSF for tuberculous meningitis and cryptococcus meningitis are established, and some differences in protein expression exist between the two.

关 键 词：双向电泳 结核性脑膜炎 隐球菌性脑膜炎 脑脊液 

分 类 号：R515.2[医药卫生—内科学]