MSCs联合PRP对兔前交叉韧带重建后腱骨愈合的影响  

Effect of MSCs in combination with PRP on tendon-bone healing of ACL reconstuction

作　　者：杨军军[1] 徐斌[1] 徐洪港[1] 涂俊[1] 李洋[1] 

机构地区：[1]安徽医科大学第一附属医院运动创伤与关节镜外科,合肥230022

出　　处：《安徽医科大学学报》2016年第3期368-372,共5页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金(编号:1208085MH157)

摘　　要：目的探讨骨隧道内局部应用自体间充质干细胞(MSCs)联合富血小板血浆(PRP)对兔前交叉韧带重建后腱-骨愈合的影响。方法 24只健康的新西兰大白兔随机平均分配为M组、P组、MP组及C组。分别在肌腱骨道内注入以生物蛋白胶为媒介的MSCs、PRP、MSCs复合PRP,C组仅注入生物蛋白胶。于术后4、8、12周3个时间点取各组兔膝关节标本切片染色,行组织学观察及分析。结果对于腱骨早期愈合有标志性意义的Sharpy样纤维在术后第4周MP组即可观察到,并随着愈合时间的延长,M组、P组及C组也相继出现Sharpy样纤维且Sharpy样纤维的量是持续增加的。各组标本在3个时间点组织形态学Buark评分差异有统计学意义(P<0.05)。结论联合使用MSCs和PRP可促进骨道肌腱早期愈合,比二者单独使用的作用强。Objective To study the effect of autologous mesenchymal stem cell （MSC） in combination with platelet rich plasma （PRP） transplantation on tendon-bone healing after anterior cruciate ligament （ACL） reconstruction. Methods Peripheral blood the harvested from rabbits to obtain MSCs. 24 adult New Zealand rabbits were random- ly divided into M, P, PM and C group（ n = 24）. Rabbits in M group were injected with MSCs via thrombin rabbits in P group were injected with PRP via thrombin, rabbits in MP group were injected with a mixture of MSCs, PRP via thrombin, while rabbits in C group were injected with only sodium hyaluronate. Four rabbits were killed 4,8 and 12 weeks after operaton and samples were collected for histological observation of tendon-bone healing. Results Scar tissue with some Sharpey＇s-like fiber components on the tendon-bone gation were observed 4 weeks in MP group. As the extension of healing interface and a close tendon-bone conju- time after operation, Sharpy＇ s like fiber could be observed in group M, group P, group C successively and the amount of Sharpy＇ s like fiber was increasing. Differences of the histomorphology Buark score of each group were significant （P 〈 0. 05 ） Conclusion MSCs in combination with PRP can promote tendon-to-bone healing, and more effective than that of single factor.

关 键 词：间充质干细胞 富血小板血浆 前交叉韧带 腱骨愈合 

分 类 号：R686[医药卫生—骨科学]