黄芪多糖对甲流HA2蛋白真核表达载体免疫效果影响的研究  

Research of astragalus polysaccharides increasing immune effect of influenza A virus HA2 eukaryotic expression vector

作　　者：褚兆苹[1] 吴淑慧[2] 刘文泰[2] 马志红[2] 徐丙元[2] 罗俊[3] 曹刚[2] 徐华洲[2] 石玉娥[2] 戴军[2] 

机构地区：[1]河北省人民医院妇产科,石家庄050051 [2]河北中医学院基础医学院,石家庄050200 [3]四川大学基础医学与法医学院微生物教研室,成都610041

出　　处：《中国免疫学杂志》2016年第2期189-192,200,共5页Chinese Journal of Immunology

基　　金：河北中医学院博士科研基金(BSZ2014002);河北省卫生厅课题(20130139)资助

摘　　要：目的:检验不同剂量黄芪多糖对甲流HA2蛋白真核表达质粒免疫大鼠效果的影响。方法:首先,将编码HA2的质粒p HA2/EGFP转染CHO细胞,通过PT-PCR和荧光检验其在真核细胞中的表达效果。然后将60只大鼠随机分为6组,分别注射p EGFP-N1、生理盐水和p HA2/EGFP加不同浓度的黄芪多糖,注射前收集大鼠血清作为对照,据最后一次注射后第36天处死各组大鼠收集各组大鼠的血清,测量血清中细胞因子IFN-γ、IL-4和血清中Ig G对不同流感毒株血凝素的反应情况。结果:p HA2/EGFP+中等剂量APS组的IFN-γ、IL-4和血清中Ig G对血凝素的反应情况均与低剂量组和阴性对照组有显著性差异(P<0.05)。p HA2/EGFP+中等剂量APS组的细胞因子水平和对血凝素反应与高剂量组无显著性差别(P>0.05)。结论:中等剂量的黄芪多糖能增强甲流HA2蛋白真核表达质粒的免疫效果。Objective：To study the astragalus polysaccharides （APS） effect on immune induced by influenza A virus HA2 eukaryotic expression vector. Methods： The HA2 encoded by the DNA vaccine vector was efficiently expressed in CHO cells, as determined by reverse transcription polymerase chain reaction （RT-PCR） and fluorescence analysis. 60 rats were divided into six groups randomly, which were immunized with normal saline, pEGFP-N1, pHA2/EGFP＋different dose of APS by intramuscular injection. The control sera were collected before injection. After injected the 36th day, sera were collected to analyzing IFN-y, IL-4 and IgG level. Results： IFN-y,IL-4 and IgG level of pHA2/EGFP＋mAPS group was different from that of pEGFP-N1 group or pHA2/EGFP ＋ lAPS group（ P 〈 0. 05 ）. Conclsion： Middle dose of APS could increase immune induced by influenza A virus HA2 eukaryotic expression vector

关 键 词：流感病毒 血凝素 IFN-Γ IL-4 

分 类 号：R373.1[医药卫生—病原生物学]