口服灭活幽门螺杆菌对新生BALB/c小鼠胃黏膜的免疫保护作用研究  

Immunoprotection of gastric mucosa in newborn mice after oral immunization with inactived H. pylori

作　　者：杨钰欣[1] 江吉富[1] 

机构地区：[1]遵义市妇幼保健院,遵义563000

出　　处：《中国免疫学杂志》2016年第2期201-204,共4页Chinese Journal of Immunology

摘　　要：目的:探索BALB/c小鼠新生期口服灭活幽门螺杆菌(H.pylori)后对胃黏膜是否具有免疫保护作用。方法:将32只BALB/c小鼠分为四组进行实验。第Ⅰ组为PBS对照组,第II组为灭活H.pylori组,第Ⅲ组为灭活H.pylori加大肠杆菌不耐热肠毒素B亚单位(LTB)组,第IV组为正常对照组。用相应抗原成分对Ⅰ、Ⅱ、Ⅲ组出生24 h以内的新生BALB/c小鼠进行灌胃免疫,末次免疫两周后,将小鼠用108CFU的幽门螺杆菌SS1株进行活菌灌胃攻击,连续攻击两天,每天两次。在攻击后的第28天,将各组小鼠处死,取胃组织进行活菌计数及组织病理学炎性评分。结果:Ⅰ、Ⅱ、Ⅲ、Ⅳ组慢性炎症评分分别为2.25±0.46、2.12±0.35、1.5±0.53、0.25±0.46,炎症活动度评分为2.12±0.35、2.12±0.35、1.37±0.52、0.25±0.46;除Ⅰ、Ⅱ组间无统计学意义外,其余组间差异均有统计学意义(P<0.05)。Ⅰ、Ⅱ、Ⅲ组细菌定植量分别为1.33±0.21、1.18±0.27、1.05±0.37×105CFU/1个胃,Ⅳ组细菌定植量<4×102CFU/1个胃,低于检测值,Ⅲ组的细菌计数低于Ⅰ、Ⅱ组,差异有统计学意义(P<0.05)。结论:BLAB/c小鼠新生期给予灭活幽门螺杆菌加LTB灌胃免疫与活菌攻击后炎症的减轻和细菌定植量的减少可能相关,具有一定的免疫保护作用。Objective： To explore the Immunoprotection of gastric mucosa in newborn mice after oral immunization with inactived H. pylori. Methods： Thirty-two BALB/c mice were randomly divided into four groups, including PBS control group （group I ）, the group of inactivated H. pylori （ group Ⅱ ）, the group of inactivated H. pylori and Escherichia cole heat-labile enterotoxin B subunit （ LTB ） （ group Ⅲ ）, and normal group （ group Ⅳ）. Newborn BLAB/c mice within 24 hours were immunized through oral gavage with corresponding antigen,Two weeks after final immunization, the mice were challenged with 1 × 10SCFU live H. pylori strain SS1, twice once day ,for 2 days. The twenty-eighth day post-challenge,all of mice were sacrificed and stomachs were removed for grades of inflammation and The counting of H. pylori colonizing. Results： The mean scores of stomach tissue inflammation of group I ,Ⅱ , Ⅲ and IV were 2. 25 ±0.46,2. 12±0. 35,1.5 ±0. 53,0. 25 ±0.46, respectively, and the score of inflammatory activity of the above four groups were 2. 12±0. 35,2. 12±0. 35,1.37 ±0. 52,0.25 ±0.46. Among these four groups, the results were statistically significant between any two groups except the result between group I and group Ⅱ Finally, H. pylori colonization quantity in the stomachs of group HI was lower than group I and groupⅡ （P〈 0. 05）,as expected,the H. pylori colonization was undeteetable in group IV. Conclusion： The oral garage with inaetived H. pylori plus LTB to newborn mice may be associate with the lightening of inflammation and the decrease of H. pylori colonizing number.

关 键 词：灭活幽门螺杆菌 LTB 新生BALB/c小鼠 免疫保护 

分 类 号：R392.9[医药卫生—免疫学]