布病患者血清microRNA-146a表达及其与抗体滴度的关系研究  

Expression pattern of MiR-146a and its correlation with antibody titers in human brucellosis

作　　者：于玖轩 徐晓阳[2] 雷霜霜 陈泽良[2] 王季秋[3] 王大力[3] 郑源强[1] 石艳春[1] 

机构地区：[1]内蒙古医科大学自治区分子生物学重点实验室,呼和浩特010058 [2]军事医学科学院疾病预防控制所,北京100071 [3]中国疾病预防控制中心鼠疫布病防治基地,白城137000

出　　处：《中国免疫学杂志》2016年第2期230-233,238,共5页Chinese Journal of Immunology

基　　金：国家"十二五"科技支撑计划项目(2014BAI13B03);国家自然科学基金项目(81171530;31272592;81401646;81460248;81260457);内蒙古自治区科技重大专项;内蒙古自治区自然科学基金(2013MS1138;2012MS1121;2015MS0820);内蒙古自治区科技计划项目(20120101;20120402;20110501);内蒙古自治区卫计委医疗卫生科研计划项目(201301035;2010018);内蒙古自治区高等学校科学研究项目(NJZY109)资助

摘　　要：目的:研究microRNA-146a在布病患者血清中表达规律及其与血清滴度的关系。方法:采用定量PCR方法,测定布病患者和健康人血清中miR-146a的表达,并分析表达水平与血清抗体水平、就诊时间等彼此的相关性。结果:利用阳性参考品建立了miR-146a定量检测工作曲线,对20例布病阳性病例和20例正常人血清miR-146a进行了检测,布病患者血清中miR-146a显著低于正常人(P<0.001),miR-146a的水平与血清抗体滴度呈负相关(P<0.05)。结论:布鲁氏菌感染后患者血清中miR-146a表达被抑制,并且患者血清抗体滴度越高,抑制程度越高。Objective： To investigate the expression pattern of microRNA-146a in Brucella patients and its correlation with antibody titers. Methods： By using real time PCR assay, expression levels of microRNA-146a in sera samples from 20 brucellosis patients and 20 healthy volunteers were analyzed. The correlation between expression level of microRNA-146a and serum antibody titers were analyzed with SPSS17. O. Results： A quantification curve of microRNA-146a was constructed with synthesized standard. Expression levels of microRNA-146a among brucellosis patients were significantly lower than those in 20 healthy volunteers （P〈0. 001 ）. For brucellosis patients ,the expression level of microRNA-146a was negatively related with antibody titers （P〈0.05）. Conclusion： Expression of miRNA-146a in brucellosis patients was significantly inhibited and negatively related with antibody titer.

关 键 词：microRNA-146a 布病 血清抗体滴度 

分 类 号：R392.7[医药卫生—免疫学]