反复种植失败患者周围血树突状细胞数量和功能分析  

Analysis of quantity and function of dendritic cells in peripheral blood of patients with repeated implantation failure

作　　者：曾勇 黄春宇 陈现 梁佩燕 刁梁辉 陈聪 张谞 尹彪 

机构地区：[1]深圳中山泌尿外科医院生殖医学中心,中山生殖与遗传研究所,深圳518045 [2]深圳中山生殖与遗传研究所,深圳518045

出　　处：《中国免疫学杂志》2016年第2期239-243,共5页Chinese Journal of Immunology

基　　金：深圳市基础研究项目(JCYJ20120829150019348)

摘　　要：目的:了解反复种植失败(Repeated implantation failure,RIF)患者周围血树突状细胞(Dendritic cells,DC)数量和功能的变化。方法:采集30例RIF患者及15例正常对照组的黄体中期周围血,采用流式细胞术(Flow cytometry,FCM)对其DC细胞功能分子及亚群进行检测。结果:与正常对照组相比,RIF患者的lin-HLA-DR+DC细胞占白细胞的比例无显著性变化(P>0.05),DC细胞表面的共刺激分子(CD80和CD86)以及免疫耐受分子CD200也无显著性变化(P>0.05)。然而,RIF患者中CD11c+CD123-m DC细胞比例显著升高(P<0.05),而CD11c-CD123+p DC细胞比例无显著性变化(P>0.05)。结论:RIF患者的周围血m DC细胞比例显著升高,其可能是影响RIF患者妊娠结局的因素之一。Objective：To evaluate the quantity and function changes of dendritic ceils （DC） in peripheral blood of patients with repeated implantation failure （RIF）. Methods： 30 patients with RIF and 15 normal controls were enrolled in this study, and the peripheral blood was collected during the mid-luteal phase. The percentage of DC subsets and the expression levels of functional molecules were assessed by flow cytometric analysis. Results： Compared with normal controls, the percentage of lin-HLA-DR＋ DC ceils accounting for leukocytes in patients with RIF was not significantly different （ P〉O. 05 ）. There were also no significant differences in the expression levels of co-stimulatory molecules （ CD80 and CD86） and immune tolerant molecules CD200 on DC cells surfaces between patients with RIF and normal controls （all P〉0. 05 ）. In addition, the percentage of CD1 l c＋ CD123- mDC accounting for DC cells was significandy increased in patients with RIF （ P〈0. 05 ）, however, the percentage of CDllc- CD123＋ pDC was similar （ P〉 0. 05 ）. Conclusion： The percentage of mDC accounting for DC cells was significantly increased in patients with RIF, which may be one of factors affecting pregnancy outcomes.

关 键 词：母胎免疫耐受 反复种植失败 周围血 树突状细胞 

分 类 号：R318.16[医药卫生—生物医学工程]