桑螵蛸炮制前后及不同药用部位对肾阳虚多尿大鼠的抗利尿作用比较  

Comparison of Antidiuretic Activity of Ootheca Mantidis before and after Processing and Its Medicinal Part against Insufficiency of Kidney-Yang and Diuresis Rats

作　　者：贾坤静 贾天柱[1,2] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中国药房》2016年第7期879-882,共4页China Pharmacy

基　　金：2011年度中医药行业科研专项项目(No.201107-007)

摘　　要：目的:比较桑螵蛸炮制前后的抗利尿作用,并探究桑螵蛸的最佳药用部位及其机制。方法:将96只大鼠随机分为空白组、模型组、阳性组、桑螵蛸生品组、桑螵蛸盐炒品组、桑螵蛸蒸品组以及生品和制品的虫卵和卵壳组,共12组,每组8只。除空白组外,其余各组大鼠均按250 mg/kg ig腺嘌呤4周,复制大鼠肾阳虚多尿模型。从第3周开始,桑螵蛸生品及制品组大鼠均按0.11g(生药)/ml ig给药,生品及各制品的虫卵和卵壳组大鼠分别按虫卵与卵壳质量比(生品为1∶2.4、盐炒品为1∶1.7、蒸品为1∶2.1)计算后ig相应药物,每天1次,连续给药4周。测定各组大鼠的尿量、体质量、肾脏指数及血清中抗利尿激素(ADH)、醛固酮(ALD)的含量。结果:与空白组比较,模型组大鼠体质量减少、肾脏指数升高、尿量增加、血清中ADH和ALD含量减少(P<0.05)。给药4周后,与模型组比较,桑螵蛸各组大鼠体质量增加、尿量减少、肾脏指数降低、血清中ADH含量增加(P<0.05),各给药组大鼠血清中ALD含量均有增加,除桑螵蛸生品组、桑螵蛸盐炒组和桑螵蛸蒸品卵壳组外其余各组大鼠血清中ALD含量差异有统计学意义(P<0.05);除ALD外,各指标变化桑螵蛸蒸品组>桑螵蛸盐炒品组>桑螵蛸生品组,且桑螵蛸蒸品卵壳组变化最大。结论:桑螵蛸经炮制后抗利尿作用增强;卵壳是桑螵蛸抗利尿作用的主要药用部位;增加血清中ADH含量可能是其缩尿作用的主要机制之一。OBJECTIVE： To compare antidiuretic activity of Ootheca Mantidis before and after processing, and to explore the best medicinal part and mechanism of Ootheca Mantidis. METHODS： 96 rats were randomly divided into blank group, model group, positive group, Ootheca Mantidis group, Ootheca Mantidis stir-fried with salt group, steamed Ootheca Mantidis group, crude product eggs and egg shell groups, processed product eggs and egg shell groups, with 8 rats in each group, 12 groups in total. Except blank group, other groups were given adenine 250 mg/kg, ig, for 4 weeks to induce kidney-yang and diuresis model. From third week, Ootheca Mantidis crude drug group and processed Ootheca Mantidis group were all given relevant medicine 0.11 g（crude drug）/ml i.g, and crude product eggs and egg shell groups and processed product eggs and egg shell groups were given rel- evant medicine, ig, once a day, by mass ratio of eggs to egg shell （cude drug 1 ： 2.4, salt stir-fried product 1 ： 1.7, steamed product 1 ： 2.1 ） for consecutive 4 weeks. The urinary volume, body weight, renal index and the serum contents of ADH and ALD were all determined. RESULTS： Compared with blank group, body weight and serum content of ADH and ALD decreased in model group, while renal index and urinary volume increased （P〈0.05）. After 4 weeks of treatment, compared with model group, body weight and serum content of ADH increased in Ootheca Mantidis groups, while urinary volume and renal index decreased （P〈 0.05）; serum content of ALD increased in treatment groups; there was statistical significance in the serum content of ALD in those groups except Ootheca Mantidis group, Ootheca Mantidis stir-fried with salt group and steamed Ootheca Mantidis group （P〈 0.05） ; except for ALD, those index were in descending order of steamed Ootheca Mantidis group〉Ootheca Mantidis stir-fried with salt group〉Ootheca Mantidis group, and steamed Ootheca Mantidis shell group had best exchange. CONCLUSIONS： The antidiuretic activity of Oot

关 键 词：桑螵蛸 抗利尿作用 炮制 药用部位 抗利尿激素 醛固酮 大鼠 

分 类 号：R285.5[医药卫生—中药学]