坦索罗辛对前列腺癌PC-3细胞增殖与凋亡的影响  

Effects of Tamsulosin on Proliferation and Apoptosis in Prostatic Cancer PC-3 Cells

作　　者：赵明[1] 金珊[1] 何锦华[1] 

机构地区：[1]天津中医药大学第一附属医院泌尿外科,天津300193

出　　处：《中国药房》2016年第7期896-899,共4页China Pharmacy

摘　　要：目的:研究坦索罗辛对前列腺癌PC-3细胞增殖与凋亡的影响。方法:以0(空白对照组)、12.5、25、50μmol/L坦索罗辛(坦索罗辛低、中、高浓度组)作用于PC-3细胞48 h后,通过MTT法检测细胞活力,Hoechst 33258染色检测细胞凋亡率,Western blot法检测核糖体Bax、B淋巴细胞瘤2(Bcl-2)蛋白表达及蛋白激酶B(Akt)、哺乳动物雷帕霉素靶蛋白(m TOR)、S6蛋白激酶(p70S6K)、4E结合蛋白1(4E-BP1)的磷酸化水平。结果:与空白对照组比较,坦索罗辛低、中、高浓度组PC-3细胞活力和Akt、p70S6K、4E-BP1磷酸化水平均降低,Bax蛋白表达水平升高(P<0.05或P<0.01);坦索罗辛中、高浓度组PC-3细胞的凋亡率升高,Bcl-2蛋白表达和m TOR磷酸化水平降低(P<0.01),上述效果均呈浓度依赖性。结论:坦索罗辛能抑制前列腺癌PC-3细胞增殖并诱导其凋亡,其机制可能与抑制Akt/m TOR信号通路激活有关。OBJECTIVE： To explore the effects of tamsulosin on proliferation and apoptosis in prostatic cancer PC-3 cells. METHODS： After treated with 0 （blank control group）, 12.5, 25 and 50 μmol/L tamsulosin （tamsulosin low, medium and high-concentration groups） for 48 h, the viability of PC-3 cells was detected by MTT method. Hoechst 33258 staining was used to detect cell apoptosis rate. Western blot was used to determine the expression level of Bax and Bcl-2 protein, and the phosphorylation level of protein kinase B （Akt）, mammalian target rapamycin （mTOR）, ribosomal S6 protein kinase （p70S6K） and 4E binding protein 1 （4E-BP1）. RESULTS： Compared with blank control group, PC-3 cells viability and the phosphorylation level of Akt, pTOS6K and 4E-BP1 decreased in tamsulosin low, medium and high-concentration groups, while expression level of Bax protein increased （P〈0.05 or P〈0.01） ; the apoptosis rate of PC-3 cells was increased in tamsulosin medium and high-concentration groups, while the expression level of Bcl-2 and phosphorylation level of mTOR were decreased （P〈0.01）, in concentration-dependent manner. CONCLUSIONS： Tamsulosin can inhibit PC-3 cells proliferation and induce cell apoptosis via blocking Akt/mTOR signal pathway.

关 键 词：坦索罗辛 前列腺癌PC-3细胞 增殖 凋亡 蛋白激酶B 哺乳动物雷帕霉素靶蛋白 

分 类 号：R965[医药卫生—药理学]