痰热清注射液联用左氧氟沙星(ig/iv)后小鼠肺组织中左氧氟沙星的分布比较  

Distribution of Levofloxacin in Lung Tissue of Mice after Intragastric and Intravenous Administration of Tanreqing Injection Combined with Levofloxacin

作　　者：邢蓉 何雄伟[2] 

机构地区：[1]重庆市东南医院药剂科,重庆400050 [2]重庆市第九人民医院药剂科,重庆400700

出　　处：《中国药房》2016年第7期923-925,共3页China Pharmacy

摘　　要：目的:比较痰热清注射液联用左氧氟沙星(ig/iv)后小鼠肺组织中左氧氟沙星的分布。方法:选用144只小鼠,随机分为对照组和实验组,每组72只。所有实验小鼠均行iv痰热清注射液(0.8 ml/kg),1 h后,对照组小鼠iv左氧氟沙星注射液,实验组小鼠ig左氧氟沙星片溶液,剂量均为80 mg/kg。分别于给药后0.05、0.083、0.17、0.5、1.0、2.0、4.0、8.0、12.0 h和0.083、0.17、0.33、0.5、1.0、2.0、4.0、8.0、12.0 h采集肺组织,每个时间点取8只小鼠。以环丙沙星为内标,采用高效液相色谱法测定肺组织中左氧氟沙星的含量,3p97软件计算药动学参数。结果:实验组和对照组小鼠肺组织中左氧氟沙星的药-时曲线均符合二室模型,t1/2β分别为(4.17±0.84)、(4.10±0.55)h,CL分别为(0.66±0.049)、(0.71±0.21)L/h,AUC0-t分别为(109.48±12.34)、(113.04±29.43)mg·h/kg,cmax分别为(38.76±1.62)、(42.28±2.03)mg/kg,组间差异无统计学意义(P>0.05)。实验组小鼠ig左氧氟沙星的绝对生物利用度为(96.85±17.39)%。结论:iv痰热清注射液后iv或ig左氧氟沙星,左氧氟沙星在小鼠肺组织中的分布相似。OBJECTIVE ： To compare the distribution of levofloxacin in lung of mice after intragastric and intravenous administration of Tanreqing injection combined with levofloxacin. METHODS： 144 mice were randomly divided into experimental group and control group, with 72 mice in each group. Both groups were given Tanreqing injection （0.8 ml/kg）. 1 h later, control group was given Levofloxacin injection （80 mg/kg） intravenously, and experimental group Levofloxacin tablet solution （80 mg/kg） intragastrically; every 8 mice were sacrificed for lung tissue collection at the time points of 0.05, 0.083, 0.17, 0.5, 1.0, 2.0, 4.0, 8.0, 12.0 h and 0.083, 0.17, 0.33, 0.5, 1.0, 2.0, 4.0, 8.0, 12.0 h, respectively. Using ciprofloxacin as internal standard, the content of levofloxacin in lung tissue was determined by HPLC, and pharmacokinetic parameters were calculated by 3p97 program. RESULTS： The concentration-time curves of levofloxacin in lung tissue were in line with two-compartment model in 2 groups. The main pharmacokinetics of experimental group and control group were as follows as t1/2β of （4.17± 0.84） h and （4.10 ± 0.55） h; CL of （0.66 ± 0.049） L/h and （0.71 ± 0.21） L/h; AUC0, of （109.48 ± 12.34） mg·h/kg and （ 113.04 ± 29.43） mg· h/kg; cmax of （38.76 ± 1.62） mg/kg and （42.28 ± 2.03） mg/kg, respectively. There was no statistical significance （P〉0.05）. Absolute bioavailability of levofloxacin was（96.85 ± 17.39）% in experimental group with intragastric administration. CONCLUSIONS： After intravenous injection of Tanreqing injection, the distribution of levofloxacin in lung tissue of mice are similiar between intragastric administration and intragastric administration.

关 键 词：左氧氟沙星 痰热清注射液 小鼠 灌胃 静脉注射 

分 类 号：R965[医药卫生—药理学]