苦豆子种子发芽检验标准化研究  

Study on Standardization of Germination Test of Sophora alopecuroides Seed

作　　者：卢有媛 李金田[1] 张延红[1] 杜弢[1] 晋玲[1] 

机构地区：[1]甘肃中医药大学药学院,兰州730000

出　　处：《中国药房》2016年第7期995-998,共4页China Pharmacy

基　　金：国家中医药管理局基金资助项目--国家基本药物所需中药材种子种苗繁育基地建设

摘　　要：目的:确定苦豆子种子发芽的最适宜条件,为制订苦豆子种子检验规程及标准化提供依据。方法:苦豆子种子以98%硫酸处理30 min+35℃温水浸泡29 h后,分别在不同温度(15、20、25、30℃)、不同发芽床(纸上、纸间、砂上、砂中)、不同光照(2 000lx光照16 h、黑暗)条件下进行处理,以发芽率、发芽势和发芽指数为指标筛选最适宜发芽条件;并在此条件下培育种子7 d,测定其发芽率。结果:不同温度对苦豆子种子发芽率影响不大,但对发芽势和发芽指数有影响,以20℃时各指标值最高;不同发芽床对各指标均有影响,以砂上处理条件下各指标值最高;光处理对苦豆子种子发芽各指标影响不大。在适宜条件下培育,种子从置床第1天起就开始萌发,第2天发芽率达90%以上,至第5天达到最大并不再增长。结论:苦豆子种子发芽的适宜培育条件为以98%硫酸处理30 min+35℃温水浸泡29 h,在砂上20℃光照培养。在种子置床第2天初次计数,第4天末次计数并统计发芽率并结束试验,此方法可作为苦豆子种子质量检验的标准化方法。OBJECTIVE： To select the optimum conditions for germination test of Sophora alopecuroides seed, and to provide reference for formulating seed test rule and standardization of S. alopecuroides. METHODS： S. alopecuroides seeds were soaked in 98% H2SO4 for 30 min and 35 ℃ warm water for 29 h, and then treated under different temperature conditions （15 ℃, 20℃, 25 ℃ and 30 ℃ ）, different germination beds （on paper, between paper, on sand, in sand） and different light conditions （2 000 lx lighting 16 h, dark）. Optimal germination condition was screened by using germination rate, germinative potential and germinative index as indicators. S. alopecuroides seeds were cultured under this condition for 7 days, and then germination rate was determined. RESULTS： Different temperatures had no significant effect on germination rate, but influenced germinative potential and germinative index; those indicators reached maximal value at 20 ℃. Different germination beds affected each indicator, and under condition of on sand, those indicators were the highest. Light treatment had no significant effect on germination indicators. Under suitable condition, the seed sprouted since first day of germination bed treatment; germination rate was more than 90% on second day, and reached maximal value on fifth day and didn＇t increase any longer. CONCLUSIONS： The suitable condition of seed germination was soaking in 98% H2SO4 30 min＋35 ℃ warm water for 29 h, on sand under light at 20 ℃. Initial count on second day of germination bed treatment and final count on forth day were analyzed statistically as well as germination rate. This method can be used as standard quality test of the seed of S. alopecuroides.

关 键 词：苦豆子 种子 发芽温度 发芽床 光照条件 发芽动态 质量检验 

分 类 号：R282.2[医药卫生—中药学]