P-选择素及P-选择素糖蛋白配体-1在大鼠深静脉血栓模型中的表达变化  

P - selectin and PSGL - 1 expression changes in rat model of deep venous thrombosis

作　　者：娄振凯[1] 彭志[2] 周如丹[1] 王兵[1] 赵学凌[1] 

机构地区：[1]昆明医科大学第一附属医院骨科,云南昆明650032 [2]大理学院附属医院外三科,云南大理671000

出　　处：《中国急救医学》2016年第2期150-154,I0001,共6页Chinese Journal of Critical Care Medicine

基　　金：国家自然科学基金资助项目（81160236）;云南省卫生科技计划项目（2014NSl41,2014NSl42）;云南省博士研究生学术新人奖项目

摘　　要：目的建立并观察大鼠深静脉血栓（deep venous thrombosis，DVT）模型不同时段血栓的形成状态，检测组织因子（tissuefactor，TF）、P-选择素（P—selectin，PS）及P-选择素糖蛋白配体-1（PSGL-1）在血液、静脉壁组织中的表达变化，探讨其与血栓形成之间的关系。方法90只SD大鼠随机分为对照组（n=10）、假手术组（n=40）和实验组（n=40）；采用下腔静脉部分瘀滞法（狭窄法）制备深静脉血栓模型，分别于造模后6h、24h、48h和72h随机处死各组10只大鼠并取材，RealTime—PCR法分别检测TF、PS及PSGL-1基因在血液中的表达变化；Westernblot法检测静脉壁组织中PSGL-1的表达变化。结果大鼠DVT模型中，对照组与假手术组各项检测指标比较差异均无统计学意义（P〉0．05）；实验组造模后6h时TF、PS及PSGL-1基因表达较对照组、假手术组明显升高（P〈0．05），24h时PSGL-1基因表达仍继续升高且差异有统计学意义（P〈0．05），随着造模时间的延长上述基因表达虽呈上升趋势，但与对照组比较差异无统计学意义（P〉0．05）。实验组PSGL-1在造模后6h、24h较对照组、假手术组表达升高（P〈0．05）。结论Ps和PSGL-1在大鼠DVT模型中呈高表达，并促进血栓形成。Objective To establish and observe the status of thrombosis formation under different time points in rat model of deep venous thrombosis （DVT）. By detecting tissue factor（ TF）, P - selectin glycoprotein ligand - 1 （ PSGL - 1 ） and P - selectin （PS） expression changes in rats＇ blood and venous wall, explore the correlation between TF, PSGL - 1 and PS with thrombosis. Methods Ninety SD rats were randomly divided into control group （n = 10 ）, sham group （n = 40 ） and experimental group （n = 40）. The deep venous thrombosis model wasmade by inferior vena cava partial stasis method （narrow）. Ten rats were executed after 6 h, 24 h, 48 h and 72 h respectively in each group, and inferior vena cava blood and venous wall were collected. Real - time PCR was used to detect the gene expressions of TF, PSGL - 1 and PS in blood. The protein expression of PSGL - 1 in venous wall was detected by Western blot. Results Each items showed no statistical difference between sham group and control group in DVT rat models （P 〉 0.05 ）. The gene expressions of TF, PS and PSGL - 1 in model group were significantly increased 6 h after modeling compared with those in sham group andcontrol group （ P 〈 0. 05 ）. The gene expression of PSGL - lwas significantly increased 24 h after modeling （P 〈 O. 05 ） ; although the gene expressions of TF and PS were increasing with time, but showed no statistical difference compared with control group （ P 〉 0.05 ）. Western blot showed that the level of PSGL - 1 protein was significantly increased 6 h and 24 h after modeling compared with sham group and control group （P 〈 0.05）. Conclusion PS and PSGL - 1 are up - regulated in rat model of deep venous thrombosis and these changes could possibly promote thrombosis formation.

关 键 词：深静脉血栓(DVT) 组织因子(TF) P-选择素(PS) P-选择素糖蛋白配体-1(PSGL-1) 

分 类 号：R392.12[医药卫生—免疫学]