苯并芘对孕早期小鼠胎盘绒毛组织的影响及槲皮素的防护作用机制  

Effect of Ba P on placental villus injury in early pregnant mice and the protective mechanism of meletin against Ba P

作　　者：高秀霞[1] 侯海燕[1] 孔祥玲[1] 刘传丽[1] 

机构地区：[1]武警后勤学院附属医院妇产科,天津300162

出　　处：《安徽医药》2016年第1期17-21,共5页Anhui Medical and Pharmaceutical Journal

基　　金：天津市应用基础与前沿技术研究计划(No 15JCQNJC2300);天津市自然科学基金资助项目(No 07J CZDJC07400)

摘　　要：目的研究苯并芘(Ba P)损伤小鼠胎盘绒毛组织细胞作用机制及探讨槲皮素(ME)的防护机制。方法选取性成熟ICR小鼠150只(100只雌鼠、50只雄鼠),随机分为5组,每组30只(20只雌鼠、10只雄鼠),雌雄分开饲养,A组予Ba P(2 mg·kg-1·d-1);B、C、D组予Ba P(2 mg·kg-1·d-1)的同时分别灌胃ME(0.5、1、2 g·kg-1·d-1);E组灌胃相同体积的0.9%氯化钠注射液与橄榄油。连续灌胃15 d后雌雄鼠同笼,第2天发现精液栓者为受孕,挑出受孕小鼠继续灌胃7 d后处死,取胎盘组织测定Bax与Bcl-2基因m RNA水平的表达、细胞色素P450(CYP450)、谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)活性。结果总共45只小鼠受孕,各组间受孕率无统计学差异(χ2=0.808,P=0.937),B、C、D组Bax基因m RNA水平表达高于E组(P<0.05),低于A组(P<0.05),且随着ME的剂量增加表达量逐渐降低(P<0.05);B、C、D组Bcl-2基因m RNA水平表达低于E组(P<0.05),高于A组(P<0.05),且随着ME的剂量增加表达量逐渐升高(P<0.05);B、C、D组中细胞色素P450酶活性明显高于E组(P<0.05),低于A组(P<0.05),且随着ME的剂量增加活性逐渐降低(P<0.05);B、C、D组中GSH-PX、SOD活性明显低于E组(P<0.05),高于A组(P<0.05),且随着剂量的增加活性逐渐升高(P<0.05)。结论 ME能通过提高抗氧化酶活力与抑制凋亡基因表达来保护苯并芘所致的胎盘绒毛组织细胞损伤,提高生育能力。Objective To study the action of injury that BaP acts on placental villus of early pregnant mice and the mechanism of mele-tin opposing BaP.Methods Totally 150 viripotent ICR mice（100 female mice,50 male mice）were randomized into five groups,ev-ery group including 30 mice（20 female mice,10 male mice）.Female and male mice were raised respectively,mice in group A were lavaged with BaP （2 mg·kg -1 ·d -1 ）,mice in group B,C,D lavaged with BaP（2 mg·kg -1 ·d -1 ）and ME（0.5,1.0,2.0 g· kg -1 ·d -1 respectively）,mice in group E lavaged with normal saline（0.9%）plusolive oil.After 15days,male mice were mated with female mice.Pregnancy was determined the next day based on the presence of a vaginal plug,then lavaging continued for another 7 days.The mice were euthanized for the detection of Bax and Bcl -2 mRNA levels and the activity of cytochrome 450,GSH -PX and SOD in placental villus.Results The total pregnancy rate was 45%,and the pregnancy rate had no statistical difference（χ2 =0.808,P =0.937）.Bax mRNA levels were higher in group B,C,D than in group E （P 〈0.05）,while lower than in group A （P 〈0.05）.With the increase of ME dose,the expression of Bax mRNA decreased （P 〈0.05）;Bcl -2 mRNA levels were lower in group B,C,D than in group E （P 〈0.05）, while higher than in group A （P 〈0.05）.With the increase of ME dose,the expression of Bcl -2 mRNA increased （P 〈0.05）.The activity of cytochrome P450 in group B,C,D was greater than in group E ,while lower than in group A（P 〈0.05）.With the increase of ME dose,the activity of cytochrome P450 decreased（P 〈0.05）.The activity of GSH -PX,SOD in group B,C,D was lower than in group E ,while greater than in group A（P 〈0.05）.With the increase of ME dose,the activity of GSH -PX,SOD increased（P 〈0.05）.Conclusion Meletin can protect the mice against placental villus injury by raising the activity of antioxidase and suppressing the cell apoptosis in the placental villus .

关 键 词：苯并芘 槲皮素 胎盘绒毛组织 凋亡 

分 类 号：R285.5[医药卫生—中药学]