番茄根内生假单胞菌的分离与鉴定  

Isolation and identification of endophytic Pseudomonas from tomato roots

作　　者：马荣琴[1] 曹毅 周俊雄[1] 叶朝松 张媞[1] 陈明波[1] 马绍城 田宝玉[1] 

机构地区：[1]福建师范大学工业微生物教育部工程研究中心生命科学学院,福建福州350108 [2]贵州省烟草科学研究院,贵州贵阳550081

出　　处：《生物技术》2015年第6期564-568,580,共6页Biotechnology

基　　金：国家自然科学基金项目("自然生境下木质纤维素微生物降解机制及其酶系的结构多样性";No.31170108);福建省自然科学基金杰青项目("水产品加工用蛋白酶基因资源的分子改造和DNA Shuffling";No.2009J06014)资助

摘　　要：[目的]通过对番茄根内生假单胞菌的分离和纯化,为研究番茄根内生假单胞菌生态学及其与植物生长之间的关系,筛选潜在的植物病害生防菌株奠定基础。[方法]以福州闽侯蔬菜大棚种植的番茄为研究对象,采用传统微生物培养技术分离纯化根内生细菌。通过16S rDNA序列同源比对与分析,鉴定和统计番茄根内生菌中可培养假单胞菌的种类和数量。[结果]在分离纯化得到的21株番茄根内生菌中,经分子鉴定其中12株为假单胞菌(Pseudomonas),序列对比和系统发育分析表明12株假单胞属可分为4个不同的类群。从统计结果来看,假单胞菌属占所培养的番茄根内生菌总数的52.1%。[结论]假单胞属细菌占番茄根可培养内生菌总数的52.1%,为优势菌,并具有较高的种类多样性。[ Objective ] The study aimed to understand ecology of endophytes inhabited in root inner tissues and their relation- ship with plant host to give a basis for developing new biological control agents through isolation and identification of endophytic Pseudomonas from tomato roots. [ Methods ] Tomato roots were collected from vegetable greenhouse in Fuzhou to isolate the en- dophytes inhabited in root tissue. By sequencing 16S rDNA gene, the sequenced were used for the identification of endophytes and construction of phylogenetie tree. [ Results] Amont the isolated 23 endophytie bacteria, 12 of them were identified as four different Pseudomonas species through RDP and phylogenetic analysis. [ Conclusion ] The results showed that Pseudomonas species were among the dominant bacterial groups with high diversity in the endophytic naicrobiome of tomato roots.

关 键 词：番茄 植物根内生菌 假单胞菌 16S RRNA基因 分子鉴定 

分 类 号：Q939.96[生物学—微生物学] S476[农业科学—农业昆虫与害虫防治]