CXCR4在乳腺癌组织芯片中的表达及其临床意义  

Expression of CXCR4 in Breast Cancer Tissue Microarray and Its Clinical Significance

作　　者：张杨[1] 王成昆[1] 彭波[1] 朱欢[1] 张志杰[2] 

机构地区：[1]南华大学医学院肿瘤研究所,湖南衡阳421001 [2]广州医科大学附属肿瘤医院肿瘤研究所,广东广州510095

出　　处：《中外医疗》2015年第33期14-16,共3页China & Foreign Medical Treatment

摘　　要：目的检测趋化因子受体4(CXC chemokinereceptortype4,CXCR4)在各种乳腺癌组织及相应正常乳腺组织中的表达并分析其临床病理意义。方法组织芯片中标本收集于2010年5月—2014年9月,应用免疫组化法检测组织芯片中204例乳腺癌组织和36例正常乳腺组织CXCR4蛋白的表达,以棕色颗粒为阳性标准计算染色强度和阳性细胞占总细胞数的百分比进行半定量处理。结果 CXCR4蛋白主要表达于细胞膜和细胞浆,在相应正常乳腺组织、小叶原位癌、导管内原位癌和浸润性导管癌中的阳性表达率依次升高,分别为8.3%(3/36)、25.0%(1/4)、10.0%(5/50)、72.0%(108/150),4组间差异有统计学意义(P<0.001);在I、II和III级以上分级的乳腺癌组织中,CXCR4蛋白的阳性表达率分别为18.2%(2/11)、80.4%(78/97)、69.8%(30/43),3组间差异有统计学意义(P<0.001);CXCR4蛋白在有转移的肿瘤组织和无转移的乳腺癌组织中的阳性率分别为88.4%(61/69)和11.1%(15/135)。结论 CXCR4在部分乳腺癌组织中高表达,且与乳腺癌组织类型及乳腺癌组织侵袭转移能力密切相关。Objective To detect the expression of CXC chemokine receptor 4 （CXCR4） in various breast cancer tissues and normal breast tissues and analyze its clinical pathological significance. Methods Immunohistochemistry was used to detect the expression of CXCR4 protein in 204 cases of breast cancer tissues and 36 cases of normal breast tissues in the tissue microarray samples collected from May 2010 to September 2014. Using brown granules as the positive standard, staining in-tensity and percentage of positive cells in the total cell number were calculated for semi-quantitative treatment. Results CXCR4 protein was mainly expressed in cell membrane and cytoplasm. The positive expression rate of CXCR4 in normal breast tissues, lobular carcinoma in situ, intraductal carcinoma in situ, infiltrating ductal carcinoma was gradually increased, which was respectively 8.3%（3/36）, 25.0%（1/4）, 10.0%（5/50） and 72.0%（108/150）, and there was a significant difference be-tween these 4 groups（P〈0.001）. The positive expression rate of CXCR4 protein in the breast cancer tissues of stage I, II, III and over III was 18.2%（2/11）, 80.4%（78/97） and 69.8%（30/43）, respectively, there was significant difference between the 3 groups （P〈0.001）. The positive rate of CXCR4 protein in metastatic tumor tissues and non-metastatic breast cancer tissues was 88.4%（61/69） and 11.1%（15/135）, respectively. Conclusion CXCR4 is highly expressed in some breast cancer tissues, and is closely related to the type of breast cancer and the invasion and metastasis of breast cancer.

关 键 词：CXCR4 乳腺癌 组织芯片 免疫组织化学法 

分 类 号：R5[医药卫生—内科学]