湖北不同地区甘薯蔓割病病原菌的分离与分子鉴定  

Isolation and Molecular Identification of Fusarium bulbigenum from Different Regions of Hubei Province

作　　者：雷剑[1] 王连军[1] 杨新笋[1] 苏文瑾[1] 柴沙沙[1] 

机构地区：[1]湖北省农业科学院粮食作物研究所/粮食作物种质创新与遗传改良湖北省重点实验室,武汉430064

出　　处：《湖北农业科学》2015年第24期6235-6237,共3页Hubei Agricultural Sciences

基　　金：国家甘薯现代产业技术体系建设项目(CARS-11-C-15);湖北省农业科技创新中心项目(2007-620-001-03);湖北省农业科学院甘薯特色学科公益性科技研究项目(2012DBA53001);科技部国际合作专项(2011DFB31620)

摘　　要：2011~2014年分别从湖北省甘薯主产区十堰市、武汉市、宜昌市等地分别采集了甘薯蔓割病(Fusarium bulbigenum)发病典型植株,在实验室分离纯化并培养,并对分离得到的真菌核糖体基因转录间隔区进行测序分析。测序结果显示目的片段长度为544 bp,武汉和宜昌的序列完全一致。从十堰扩增出来的序列的385位置是C而武汉和宜昌扩增的结果该位置是T,说明引起湖北省甘薯蔓割病的甘薯镰孢菌存在多态性。From the city of Shiyan, Wuhan, Yichang, 3 Fusarium bulbigenum pathogens were separated and purified, by Molecular identified F. bulbigenum from different regions of Hubei province. The test results showed that the length of the tar- get fragment was 544 bp, and the sequence of Wuhan and Yichang was the same. From Shiyan amplified sequence of 385 position was C and Wuhan and Yichang amplification results of the location was T, molecular indicating that the F. bulbi- genurn from different regions of Hubei province presenced polvmo^hism.

关 键 词：甘薯 甘薯蔓割病(Fusarium bulbigenum) 病原菌 分离 分子鉴定 

分 类 号：S432.44[农业科学—植物病理学]