猪TCAP基因转录因子的筛选与鉴定  

Screening and Identification of Transcription Factor of Pig TCAP Gene

作　　者：乔木[1] 程碧军[2] 武华玉[1] 吴彪 刘贵生[1] 李良华[1] 吴俊静[1] 彭先文[1] 梅书棋[1] 

机构地区：[1]湖北省农业科学院畜牧兽医研究所,武汉430064 [2]湖北省农业科学院农业经济技术研究所,武汉430064 [3]通城县畜牧兽医局,湖北通城437400

出　　处：《湖北农业科学》2015年第24期6299-6302,共4页Hubei Agricultural Sciences

基　　金：湖北省农业科技创新中心项目(2015-620-004-001);国家生猪产业技术体系项目(CARS-36);国家科技支撑计划项目(2011BAD28B01-13);湖北省重大人才工程高端人才引领培养计划项目

摘　　要：克隆了猪TCAP基因1 662 bp的启动子序列,序列分析发现其启动子区域存在Myo D、Myo G、MEF2转录因子结合位点,并构建了3个转录因子超表达载体,分别与TCAP基因启动子载体共转染PK细胞。结果表明,3个转录因子使TCAP基因启动子活性升高,均正调控TCAP基因的表达。In this study,1 662 bp sequence of pig TCA P gene promoter was cloned. Some specific transcription factor （MyoD, MyoG and MEF2） binding sites were found in TCA P gene promoter by bioinformatics analysis. Over-expression vectors of MyoD,MyoG and MEF2 were successfully constructed and transfected with promoter sequences, respectively. Detection analysis showed that TCA P promoter activity was significantly higher. The results indicated that transcription factors MyoD,MyoG and MEF2 could bind to the TCA P promoter sequences, and enhance its activity.

关 键 词：猪 TCAP基因 转录因子 表达调控 

分 类 号：S828[农业科学—畜牧学] Q78[农业科学—畜牧兽医]