膝骨性关节炎患者关节液中MMP-7、MMP-9、TIMP-1、TNF-α的表达及意义  

The level and the important significance of MMP-7, MMP-9, TIMP-1, and TNF-α in synovial fluid of patients with knee osteoarthritis

作　　者：鲁海[1] 卢超[1] 张嘉良[1] 刘文刚[1] 倪新玲[1] 

机构地区：[1]广东省第二中医院骨一科,广东广州510095

出　　处：《中国医药科学》2015年第22期11-15,共5页China Medicine And Pharmacy

摘　　要：目的观察并探讨膝关节骨性关节炎患者膝关节液中MMP-7、MMP-9、TIMP-1、TNF-α水平与病变程度的关系。方法膝关节骨性关节炎患者97例,根据患者的X线片,把OA的严重程度分为0~Ⅳ级,并进行WOMAC骨关节炎指数评分,检测膝关节液中MMP-7、MMP-9、TIMP-1蛋白表达量及TNF-α水平,以健康志愿者10例为正常对照组。结果骨性关节炎患者膝关节液中MMP-7、MMP-9、TIMP-1、TNF-α水平均高于正常对照组（P〈0.05）,随着病变程度加重,膝关节液中MMP-7、MMP-9、TIMP-1、TNF-α水平显著增加。结论 MMP-7、MMP-9、TIMP-1、TNF-α水平在骨性关节炎患者膝关节液中均升高,与病变程度密切相关,检测这些指标有助于对膝关节骨性关节炎病情的判断。Objective To explore the relationship between the levels of synovial fluid matrix metalloproteinase-7（MMP-7）, matrix metalloproteinase-9（MMP-9）, matrix metalloproteinase inhibitor-1（TIMP-1）, tumor necrosis factor-α with the severity of knee osteoarthritis（KOA）. Met hods According to the X-ray, 97 knee osteoarthritis patients, were divided into 0 to IV stages based on the severity of KOA; and WOMAC osteoarthritis index scores were evaluated. Another 10 healthy volunteers were selected as the control group. MMP-7, MMP-9, TIMP-1 and TNF-α level in knee joint fluid were all detected. Results MMP-7, MMP-9, TIMP-1 and TNF-α levels of synovial fluid in KOA group were significantly higher than those in the control group（P〈0.05）.The levels of MMP-7,MMP-9, TIMP-1and TNF-α in the synovial fluid were significantly increased with the aggravation of the lesions. Conclusion MMP-7, MMP-9,TIMP-1 and TNF-α level in the knee joint fluid increased in patients with osteoarthritis and were closely related with the extent of the lesions; so detection of these indicators will be helpful to judge the condition of osteoarthritis of the knee.

关 键 词：骨性关节炎 膝关节 基质金属蛋白酶 肿瘤坏死因子Α 

分 类 号：R684[医药卫生—骨科学]