酶-热水浸提法提取藜麦麸水溶性非淀粉多糖工艺研究  

Extraction process of water-soluble non-starch polysaccharides from Chenopodium quinoa willd bran by enzyme-water method

作　　者：申瑞玲[1] 张文杰[1] 董吉林[1] 

机构地区：[1]郑州轻工业学院食品与生物工程学院,河南郑州450001

出　　处：《轻工学报》2016年第1期29-34,共6页Journal of Light Industry

基　　金：国家自然科学基金项目(31271854);郑州轻工业学院研究生科技创新基金项目(2014025)

摘　　要：以藜麦麸为原料,采用酶-热水浸提法对藜麦麸水溶性非淀粉多糖(NSP)提取工艺进行研究.利用苯酚-硫酸法测定NSP的得率,通过单因素试验和正交试验确定最佳提取工艺条件:料液比(w/v)为1∶16,复合多糖酶添加量为2.0 mg/g,浸提温度为90℃,浸提时间为100 min.该试验条件下藜麦麸水溶性NSP得率为7.55%.与其他提取方法相比,该法提取水溶性NSP的得率较高,对藜麦麸的利用更加充分,且浸提溶剂为水,在提取过程中没有强酸强碱性废液产生,不会造成环境污染.Using Cheopodium quinoa willd bran, the extraction process of water-soluble non-starch polysaccharides （NSP） was studied by enzyme-water method. Phenol-sulfate method was used to measure the yield of NSP. The optimal extraction conditions were determinated through single factor assay and orthogonal assay. The results showed that the optimal extraction conditions were material-liquid ratio（w/v） 1 ： 16, the addition of viscozyme 2.0 mg/g, extraction temperature 90 ℃, extraction time 100 min. Under the experiment conditions, the yield of NSP was 7.55%. Compared with other extraction methods, the extraction yield of water-soluble NSP was higher, to the use of Cheopodium quinoa willd bran more fully. And because extraction solvent was water, there was no strong acid alkali waste liquid generated in extraction process, which would not cause environmental pollution.

关 键 词：藜麦麸 水溶性非淀 粉多糖 酶-热水浸 提法 

分 类 号：TS210.9[轻工技术与工程—粮食、油脂及植物蛋白工程]