外源绿原酸对大豆下胚轴不定根形成的影响  

Effect of Exogenous Chlorogenic Acid on Adventitious Rooting of Soybean Hypocotyl

作　　者：刘江[1] 陈中说[1] 吴春[1] 吴海军[1] 张晨州 陈建华[1] 邓俊才[1] 秦雯婷[1] 杨文钰[1] 陈兴福[1] 

机构地区：[1]四川农业大学农学院农业部西南作物生理生态与耕作重点实验室,成都611130

出　　处：《天然产物研究与开发》2016年第2期262-265,共4页Natural Product Research and Development

基　　金：国家自然科学基金青年科学基金(31401329);中国博士后科学基金面上项目(2014M560724)

摘　　要：以大豆下胚轴为试验材料,研究了绿原酸（CGA）及其与植物生长调节剂（IAA、IBA）共同作用对大豆下胚轴不定根发生的影响。结果表明,当CGA施用量达到200μmol/L及400μmol/L时,大豆下胚轴生根率分别显著降低了17.8%和26.7%,平均根长分别显著降低了18.7%和35.4%;当植物生长调节剂施用量为2μmol/L时,可显著缓解CGA（100μmol/L）对大豆不定根的抑制作用,使其生根率分别提高12.5%和20.0%,随着生长素施用量的进一步增加（10~50μmol/L）,其生根率显著下降;大豆不定根培养24 h内,对照下胚轴基部木质素相对含量降低,CGA处理大豆的木质素相对含量升高。高浓度CGA对大豆不定根形成具有抑制作用,其通过与木质素、生长素的共同作用,实现对大豆不定根发生的调控。The effect of chlorogenic acid （CGA） and IAA/IBA on adventitious rooting of soybean hypocotyl was explored in this study. The results indicated that after treatment with 200 and 400 μmol/L CGA, rooting rate of soybean hypocotyl significantly decreased （ 17.8% and 26.7%, respectively）, average root length significant decreased （ 18.7% and 35. 4% ,respectively） ;The inhibition effect of CGA on the adventitious rooting of soybean hypocotyl can be remitted by application of 2 μmol/L IAA/IBA, their rooting rate improved significantly （ 12.5 % and 20.0%, respectively） ;with the increasing doses of IAA/IBA applications （10 - 50 μmol/L）, the rooting rate significant decreased. Lignin content of soybean hypocotyl decreased in the first 24 h after treatment. The lignin content of samples suffered from 100 μmol/L CGA,increased inversely. High concentration of CGA treatment inhibited the growth of adventitious rooting of soybean. Low concentration of IAA/IBA treatment can remit the inhibition, high level of IAA/IBA treatment increased inhibition. In conclusion, CGA itself might not directly involve in organogenesis, but, might be related to a balance between IAA/ IBA and lignin that control organogenesis of adventitious rooting in soybean hypocotyl.

关 键 词：绿原酸 大豆下胚轴 不定根 抑制 

分 类 号：Q946.8[生物学—植物学]