银杏黄酮对非酒精性脂肪肝模型小鼠的作用及NF-κB在其机制中的相关性分析  

Effect of Ginkgo Flavone on Nonalcoholic Fatty Liver Disease in Mice and the Correlation Analysis on NF-κB in the Action Mechanism

作　　者：梁志清[1] 白纪红[1] 赵日红[1] 刘艳华[1] 李丽梅[1] 林秋香[1] 

机构地区：[1]桂林医学院附属医院,桂林541001

出　　处：《天然产物研究与开发》2016年第2期277-282,共6页Natural Product Research and Development

基　　金：国家自然科学基金(81260078);广西自然科学基金(2012GXNSFAA276038;2014GXNSFAA118152);广西医药卫生自筹经费计划(Z2015367)

摘　　要：本文观察银杏黄酮对非酒精性脂肪性肝(NAFLD)模型小鼠的作用,并分析NF-κB与小鼠肝脏脂肪变性及血清丙氨酸氨基转移酶(ALT)的相关性。170只昆明小鼠随机分为正常对照组、模型组、银杏黄酮高[300mg/(kg·d)]、中[150 mg/(kg·d)]、低[75 mg/(kg·d)]剂量组。采用高脂饲料构建非酒精性脂肪肝小鼠动物模型,分别于给药后4周、8周和12周末处死,观察肝脏病理变化,计算肝指数,检测血清总胆固醇(TC)、ALT、TNF-α以及肝组织NF-κB的表达。各剂量银杏黄酮组小鼠肝指数,血清TC、ALT和TNF-α水平,肝组织NF-κBp65的表达量比模型组显著降低,肝脏脂肪变性明显减轻(P<0.05或P<0.01);肝组织NF-κBp65相对表达量与肝脏脂肪变性程度及血清ALT含量呈正相关(r>0、P<0.01或P<0.05)。实验结果表明:银杏黄酮可降低NAFLD小鼠血脂、减轻肝脏脂肪变性、改善肝功能指标及肝脏大体形态,且有一定的剂量和时间依赖性;NF-κB上游炎症通路可能是其作用的主要机制。In this study, the effect of Ginkgo Flavone on nonalcoholic fatty liver disease （NAFLD） in mice was investiga- ted, and the correlation between NF-κB and the level of hepatic steatosis or serum ALT was analyzed. 170 KM mice were randomly divided into 5 groups：control group, model group, high [ 300 mg/（ kg · d） ], medium [ 150 mg/（ kg· d） ] and low [75 mg/（kg · d） ] dosage of Ginkgo FIavone groups. High fat diet was used to establish the animal model. The pathological changes of liver, liver index, the serum TC, ALT,TNF-ct and NF-KB in hepatic tissue were observed after 4, 8 and 12 weeks of administration. Compared with the model group：the level of liver index, serum TC, ALT, TNF-α and the expression of NF-KBp65 in the Ginkgo Flavone groups were dramatically decreased. The hepatic steatosis was milder （P 〈 0.05 or P 〈 0.01 ）. The correlation analysis showed that the NF-KBp65 in hepatic tissue was positively correlated with the hepatic steatosis and the serum ALT （r 〉0,P 〈 0.01 or P 〈 0.05 ）. These results showed that Ginkgo Flavone can decrease the serum lipid and hepatic steatosis ,improve the index of liver function and liver histolomorph in the time and dosage dependent manner. NF-κB related pathway might be the main mechanism of the effect.

关 键 词：银杏黄酮 非酒精性脂肪性肝病 核因子-ΚB 

分 类 号：R96[医药卫生—药理学]