刀豆球蛋白A刺激对人外周血T淋巴细胞的增殖作用  

Proliferation of Human Peripheral Blood T Lymphocytes Stimulated by Concanavalin A

作　　者：张娜[1] 封颖璐[2] 

机构地区：[1]青岛大学医学院中西医结合中心,山东青岛266003 [2]解放军第401医院中医科,山东青岛266071

出　　处：《临床医学工程》2016年第2期155-157,共3页Clinical Medicine & Engineering

基　　金：国家自然科学基金项目(项目编号:81173388)

摘　　要：目的探讨刀豆球蛋白A刺激人外周血T淋巴细胞增殖的最佳浓度。方法以人外周血制备T淋巴细胞,与刀豆球蛋白A共同培养后,采用CCK-8法检测T淋巴细胞的增殖情况。结果不同浓度的刀豆球蛋白A对人外周血T淋巴细胞的刺激效果不同,以20μg/m L时刺激增殖效果最好。大于20μg/m L时,刀豆球蛋白A表现出对人外周血T淋巴细胞的抑制作用。结论刀豆球蛋白A对人外周血T淋巴细胞的增殖具有双重作用,且在20μg/m L时刺激增殖效果最好。Objective To investigate the optimum concentration of concanavalin A in stimulating the proliferation of T lymphocytes of human peripheral blood. Methods T Lymphocytes were prepared from human peripheral blood, and the proliferation of T lymphocytes was determined by CCK-8 after jointly culturing with concanavalin A（Con A）. Results The stimulative effect depended on the concentration of concanavalin A, and 20 μg/m L Con A had best efficacious for the proliferation of human peripheral blood T lymphocytes.When the concentration of concanavalin A 〉20 μg/m L, the proliferation of human peripheral blood T lymphocytes was inhibitted.Conclusions Concanavalin A plays a dual role in the proliferation of human peripheral blood T lymphocytes, and 20 μg/m L Con A has best proliferative effect.

关 键 词：刀豆球蛋白A 淋巴细胞增殖 CCK-8 

分 类 号：R392-33[医药卫生—免疫学]