降压通络方对高血压肾损害大鼠肾脏及血浆ADM的影响  

Effects of Jiangya Tongluo Formula(降压通络方) on Adrenomedullin in Kidneys and Plasma in Rats with Hypertensive Nephrosclerosis

作　　者：宋林梅 韩琳[1] 郭一[2] 张晓宇[2] 罗燕妮[2] 迟笑怡[2] 

机构地区：[1]北京中医药大学基础医学院,北京100029 [2]北京中医药大学东方医院,北京100078

出　　处：《中医药导报》2016年第3期5-8,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(NO.811734071);北京市薪火传承郭士魁研究室项目(2011-SZ-A-27)

摘　　要：目的:观察降压通络方对高血压肾损害大鼠肾脏及血浆肾上腺髓质素(adrenomedullin,ADM)表达的影响,探讨降压通络方对高血压肾损害大鼠肾脏的保护机制。方法:将16周龄自发性高血压大鼠(spontaneously hypertension rat,SHR)随机分为模型组、缬沙坦组、降压通络方组,同时设16周龄正常血压大鼠(wistar kyoto,WKY)为正常对照组。缬沙坦组、降压通络方组分别给予缬沙坦、降压通络方灌胃干预,模型组及正常对照组大鼠给予等容积的生理盐水灌胃,连续8周。分别于给药前、给药4周、给药8周末测定大鼠血压;给药4周、8周末,检测血肌酐、尿素氮及肾脏、血浆ADM的含量。结果:给药4周、8周后,降压通络方组、缬沙坦组大鼠血压、肌酐、尿素氮均低于模型组(P<0.05或P<0.01);而大鼠肾脏ADM含量高于模型组(P<0.05或P<0.01);给药4周后,模型组及降压通络方组、缬沙坦组大鼠血浆ADM明显高于正常对照组,8周后模型及降压通络方组、缬沙坦组血浆ADM低于正常对照组,但两个时间点模型组与降压通络方组、缬沙坦组之间比较,差异无统计学意义。结论:降压通络方可降低高血压肾损害大鼠血压,保护肾功能,可能机制为上调肾脏局部ADM的表达。Objective： To observe the dffects of Jiangya Tongluo Formula on adrenomedullin in kidney and , valsartan group and Jiangyatengluo group （JYTL group）, and Wistar Kyoto rats were used as control group. JYTL group and valsartan group were given Jiangyatongluo Formula and vatsartan respectively by gavage; the model group and WKY group were given the same volume physiological saline, for 8 weeks. The tail arterial pressure was detected before a6dministration 4weeks and 8 weeks after administration respectively. The serum creatinine, blood Urea nitrogen levels, content of ADM in kidney and plasma were estimated after each group was sacrificed, 4 weeks and 8 weeks after administration respectively. Results： The JYTL and valsartan groups showed lower rats tail arterial pressui-e, serum creatinine level and blood urea nitrogen than the model group （P〈0.05 or P〈0.01）, and higher content of ADM than the model group（P〈0.05 or P〈0.O1）, 4weeks and 8 weeks after administration. And compared to control group, the content of ADM in plasma was significantly higher in model, JYTL and valsartan groups, 4weeks after administration, bat lower at the end 6f treated 8 weeks, and there were no statistically significant between JYTL and valsartan groups and models at the two time points. Conclusion： Jiangyatongluo Formula could reduce the blood presstlre of rats with hypertensive nephrosclerosis and protect renal function and the protective function of JYTL may be throngh up-regalation endogenous ADM level in the kidneys.

关 键 词：高血压肾损害 降压通络方 肾上腺髓质素 大鼠 

分 类 号：R285.5[医药卫生—中药学]