土龙祛疣洗剂对尖锐湿疣皮损内cFLIP-TRAIL凋亡通路影响的研究  

The Effects of Tulong Quyou Lotions(土龙祛疣洗剂) on cFLIP-TRAIL Apoptosis Pathway in the Lesions of Condyloma Acuminatum Patients

作　　者：吴志洪[1] 钟江[1] 岳天天[1] 徐张杰[1] 苏传丽[1] 郑恩金[1] 

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023

出　　处：《中医药导报》2016年第3期13-16,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(NO.81360599);广西自然科学基金面上项目(NO.2013GXNSFAA019148);广西壮族自治区卫生厅课题(GZZY13-17)

摘　　要：目的:探讨土龙祛疣洗剂对尖锐湿疣患者皮损内c FLIP-TRAIL凋亡通路的影响。方法:将57例尖锐湿疣患者随机分为治疗组29例和对照组28例;治疗组予土龙祛疣洗剂治疗,对照组予液氮冷冻治疗,治疗6周后观察疗效及复发情况。收集皮损标本,分别采用原位杂交法、免疫组化法检测皮损内HPV病毒感染亚型及c FLIP-TRAIL和CXCL10/IP10蛋白的表达。结果:两组临床疗效比较,差异无统计学意义(P>0.05),治疗组复发率低于对照组。治疗组患者治疗后皮损中c FLIP-TRAIL明显上调(P<0.05),可能与CA预后密切相关;治疗组患者治疗前后表皮Bax、Bcl-2蛋白变化不明显(P>0.05);患者皮损中CXCL10/IP10与c FLIP-TRAIL指数呈显著正相关(r=0.7715,P<0.05;r=0.8088,P<0.05)。结论:土龙祛疣洗剂可能通过上调c FLIP-TRAIL的表达、增强CXCL10/IP10活性来发挥抗病毒的治疗作用。Objective： To investigate the effect of Tulong Quyou Lotions （土龙祛疣洗剂） on cFLIP-TRAIL apoptosis pathway in the lesions of condyloma acuminatum patients. Methods： The total of 57 cases of condyloma acuminatum patients were randomly divided into treatment group with 29 cases, and control group with 28 cases. The patients were treated by Tulong Quyou Lotions in treatment group, and liquid nitrogen frozen in control group. The efficacy and recurrence condition were observed in 6 weeks; the lesion specimens were col- lected, and HPV virus subtypes, cFLIP-TRAIL and CXCL10/IP10 proteins were detected by the methods of in situ hybridization and immunohistochemistry. Results： The data of clinical efficacy of the two groups showed no significant difference （P〉0.05）, and the treatment group showed lower recurrence rate. The cFLIP-TRAIL proteins were increased in treatment group （P〈O.05）, which may be related to the prognosis of CA. However, the proteins of Bax and Bcl-2 did not change significantly （P〉0.05）. The proteins of CXCL10/IP10 showed a significant positive correlation to the pathway of cFLIP-TRAIL（r=0.7715, P〈0.05; r=0.8088, P〈0.05）. Conclusion： The Tulong Quyou Lotions shows an immunomodulatory effect in CA tissues by upregnlating the expression of cFLIP-TRAIL proteins and enhancing the antiviral activity of CXCL10/IP10.

关 键 词：尖锐湿疣 人类乳头瘤病毒 凋亡 土龙祛疣洗剂 TRAIL CXCL10 

分 类 号：R285.5[医药卫生—中药学]