青藤碱降低兔膝骨关节炎模型瘦素含量及其受体表达  

Sinomenine down-regulates expression of leptin and its receptor in rabbit knee osteoarthritis model

作　　者：郑洁[1] 王瑞辉[1] 杨威[2] 寇久社[3] 

机构地区：[1]陕西中医药大学针灸推拿学院,陕西咸阳712046 [2]陕西中医药大学中医系,陕西咸阳712046 [3]陕西中医药大学第二附属医院,陕西咸阳712046

出　　处：《中国骨质疏松杂志》2016年第2期145-149,共5页Chinese Journal of Osteoporosis

基　　金：陕西省中医康复学重点学科建设项目(陕中医药发[2011]46号);陕西省教育厅科学研究项目(14JK1209);陕西中医学院创新基金培育项目(14XJZR27)

摘　　要：目的观察青藤碱关节腔注射对兔膝骨关节炎模型血清及关节液瘦素水平以及关节软骨瘦素受体(Ob-Rb)表达的影响。方法 38只新西兰大白兔随机分为空白组、模型组、透明质酸钠组和青藤碱组。空白组不予任何处理,模型组、透明质酸钠组和青藤碱组通过木瓜蛋白酶关节腔注射法诱导膝骨关节炎模型后,分别用生理盐水、透明质酸钠和青藤碱干预5周,双抗体夹心酶联免疫吸附(ELISA)法检测血清及关节液瘦素含量,实时荧光定量PCR(q PCR)法检测Ob-Rb的m RNA表达,Western blot法检测Ob-Rb的蛋白表达。结果模型组和透明质酸钠组血清、关节液瘦素水平较空白组均显著升高(P<0.05),青藤碱组血清、关节液瘦素水平明显低于模型组(P<0.01)和透明质酸钠组(P<0.05);模型组Ob-Rb的m RNA和蛋白表达较空白组均显著升高(P<0.05,P<0.01),青藤碱组Ob-Rb的m RNA和蛋白表达明显低于模型组(P<0.05,P<0.01)。结论青藤碱膝关节腔注射可降低兔膝骨关节炎模型血清及关节液瘦素水平,下调关节软骨Ob-Rb的表达。Objective To observe the effect of intra-articular injection of sinomenine （SIN） on leptin in serum and articular synovium as well as expression of leptin receptor （ Ob-Rb ） in rabbit knee osteoarthritis model. Methods A total of 38 New Zealand white rabbits were randomly divided into 4 groups ： control group, model group, hyaluronate group, and sinomenine group. Knee osteoarthritis model was established with intra-articular injection of papain enzyme in model group, hyaluronate group, and sinomenine group and treatment with saline, sodium hyaluronate, and sinomenine, respectively, lasted for 5 weeks except for control group. ELISA method was used to detect the level of leptin in rabbit serum and articular synovium, qPCR was used to detect the mRNA level of Ob-Rb. Western blotting was used to detect the protein level of Ob-Rb. Results The level of leptin in serum as well as in articular synovium in model group was significantly higher than that in control group （ P 〈 0. 05 ）, and the levels of serum and articular synovium leptin in sinomenine group was significantly lower than those in model group （ P 〈 0.01 ） and hyaluronate group （ P 〈 0.05 ）. mRNA and protein expression of Ob-Rb in model group were higher than those in control group （ P 〈 0. 05, P 〈 0.01 ）. mRNA and protein expression of Ob-Rb in sinomenine group were lower than those in model group （ P 〈 0.05, P 〈 0. 01 ）. Conclusion Intra-articular injection of sinomenine can reduce the level of leptin in serum and articular synovium and down-regulate the increased expression of Ob-Rb in cartilage in rabbit knee osteoarthritis model.

关 键 词：青藤碱 骨关节炎 瘦素 瘦素受体 

分 类 号：R683[医药卫生—骨科学]