棉花黄萎病菌致病相关基因的分离及敲除载体的构建  

Isolation of the pathogenicity-related gene from Verticillium dahliae and construction of knockout vector in cotton

作　　者：李小萍[1] 汪敏[1] 赵杨[1] 丁胜利[1] 李洪连[1] 马宗斌[2] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002 [2]河南农业大学农学院,河南郑州450002

出　　处：《河南农业大学学报》2015年第6期787-793,共7页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(30700520);农业部公益性行业科技专项(201503109);河南省教育厅科学技术研究重点项目(14A210025);河南省棉花产业技术体系建设专项资金资助(S2013-07-G04)

摘　　要：利用分生孢子蘸根法筛选棉花黄萎病菌T-DNA插入转化体库,获得了1个致病力减弱的突变体V546,该突变体在PDA平板上不产生微菌核。利用hi TAIL-PCR技术,获得了突变体V546 T-DNA插入的侧端序列。序列比对分析表明,该突变体T-DNA插入位点在大丽轮枝菌基因VDAG_07282.1的编码区。The pathogenicity of V. dahliae T-DNA transformants on the cotton variety Yinshan 1 seeding was tested by dipping roots with V. dahliae spore suspension,and a pathogenicity defective mutant V546 was obtained,indicating microsclerotia development defectively on PDA. The T-DNA insertional flanking sequence of mutant V546 was isolated by hi TAIL-PCR,which was integrated in the V. dahliae gene VDAG_07282. 1 coding sequence by genome sequence alignment.

关 键 词：棉花黄萎病 大丽轮枝菌 致病相关基因 侧端序列 敲除载体 

分 类 号：S435.6[农业科学—农业昆虫与害虫防治]