CB1大麻素受体激动剂抑制基质金属蛋白酶参与脊髓损伤后血-脊髓屏障通透性调节  

Involvement of CB1 cannabinoid receptor agonist in the permeability of blood spinal cord barrier after acute spinal cord injury in rat model

作　　者：董宝铁[1] 李泓[1] 费良健[1] 王岩峰[2] 

机构地区：[1]沈阳市第四人民医院骨一科,辽宁沈阳110031 [2]中国医科大学附属第一医院运动医学及关节外科,辽宁沈阳110001

出　　处：《临床军医杂志》2015年第9期906-908,共3页Clinical Journal of Medical Officers

基　　金：国家青年自然科学基金项目(81100924)

摘　　要：目的：探讨CB1大麻素受体激动剂在大鼠脊髓损伤后对血-脊髓屏障通透性调节的作用。方法将150只雌性SD大鼠随机分为假手术组（ Sham组）、脊髓损伤组（ SCI组）和CB1激动剂处理组（ ACEA组）。采用改良Allen法建立T9脊髓损伤实验动物模型。 Sham组仅行T9椎板切除术,SCI组和ACEA组以30 g·cm致伤力制作模型。 ACEA组建模成功后,每日腹腔给药ACEA 3mg/（kg·d）；Sham组和SCI组以生理盐水代替。建模术后12、24、72 h分时段处死动物,取T8～T10脊髓节段,Evans蓝含量测定法检测SCI后血-脊髓屏障通透性变化,定量RT-PCR法检测脊髓组织基质金属蛋白酶9（MMP9）表达水平。结果 Sham组脊髓通透性无改变,脊髓组织中无Evans蓝渗入,ACEA组Evans蓝通过血-脊髓屏障渗漏至脊髓,但渗漏量明显低于SCI组。 ACEA组MMP9表达水平显著低于Sci组。结论 CB1受体激动剂ACEA能降低Allen′s大鼠脊髓损伤模型血-脊髓屏障的破坏,其作用机制可能与MMP9的表达下调相关。Objective To investigate whether the CB1 cannabinoid receptor agonist has regulating effect on permeability of blood spi-nal cord barrier（ BSCB） after spinal cord injury（ SCI） in rat model. Methods Totally 150 female SD rats were randomly divided into three groups,including sham operation group（Sham group）,spinal cord injury group（SCI group）and ACEA treatment group（ACEA group）. Modified Allen′s method was carried out at T9 level spinal segment for SCI group and ACEA group to induce acute SCI. While Sham group only underwent laminectomy. Rats in ACEA group were treated with ACEA 3 mg/（ kg·d） after surgery until killed. After modeling,the animals were sacrificed at 12,24 and 72 hours,and the level of permeability for BSC was detected by Evans blue assay at T8-T10. The expression level of MMP9 was detected by quantitative RT-PCR method. Results There was no change in the permeabil-ity of BSCB for the Sham group,no Evans blue in the spinal cord tissues,and the Evans blue in the ACEA group was significantly lower than that in SCI group. The expression level of MMP9 in ACEA group was significantly lower than that in SCI group. Conclusion CB1 receptor agonist ACEA can reduce the damage of blood spinal cord barrier in rats with spinal cord injury induced by Allen′s,and its mechanism may be related to the down-regulated expression of MMP9 expression.

关 键 词：血脊髓屏障 大麻素1 ACEA 基质金属蛋白酶9 脊髓损伤 

分 类 号：R687[医药卫生—骨科学]