醉茄素－A抗肺鳞癌细胞上皮间质转化的相关研究  

作　　者：刘凤娟 于瀚宇 邢乃娇 孙荣丽 

机构地区：[1]山东省青岛市中心医院,山东青岛266042

出　　处：《中国药业》2015年第B12期3-5,共3页China Pharmaceuticals

基　　金：山东省青岛市卫生局立项课题,项目编号：2012-WSZD083.

摘　　要：目的探讨醉茄素－A（WFA）对肺鳞癌细胞上皮间质转化（上皮标志物E—cadherin和间质标志物Vimentin的表达）的影响，以及Notch受体拮抗剂对肺鳞癌细胞上皮间质转化（EMT）的作用及抗肺鳞癌的作用机制。方法体外培养肺鳞癌细胞（SK—MES－1）。用终浓度分别为0（对照组），10，20，40μg／mL的醉茄素－A组处理SK—MES－1细胞24h后，于相差显微镜下观察细胞的一般形态结构、脱落情况等；M1Tr法检测细胞的生长活性；酶联免疫法（ELISA）和RT—PCR法检测上皮标志物E—cadherin和间质标志物Vimentin的表达。结果不同浓度的醉茄素－A均能抑制SK—MES－1细胞增殖，其抑制率与作用浓度相关；ELISA结果提示，不同浓度WFA作用SK—MES－1细胞24h后，与对照组比较，浓度为20，40μg／mLWFA组，E—cadherin较对照组表达增加，与浓度呈正相关：Vimentin较对照组表达减少，差异有统计学意义（P〈0．05）；其余浓度WFA组无明显变化；RT—PCR结果显示，在质量浓度为20，40仙g／mL醉茄素－A试验组E—cadherinmRNA表达增加，VimentinmRNA表达减少，差异有统计学意义（P〈0．05）。结论醉茄素－A能抑制肺鳞癌细胞的生长并抑制其上皮一间质转化（EMT）过程，醉茄素－A可能通过抑制肺鳞癌细胞发生上皮间质转化从而抑制肿瘤生长。Objective To investigate about the relationship between withaferin-A treat lung squamous carcinoma cell and Epithelial Senchymal transition（EMT）; To study the effect of Notch receptor antagonist on epithelial mesenchymal transition （EMT） and the possible mechanism of anti lung squamous cell carcinoma.Methods Culture lung adenocarcinoma（SK-MES-1 ）in vitro,and respec- tively use WFA final coneentratino 0 （control group）,10,20,40 Ixg/mL group to cells after 24 h,then the cells of structure and loss situation were observed by the phase contrast microscope morphological structure; Determined by MTT method to detect the cell growth activity; the expression of Vimentin and E-cadherin were detected by enzyme linked immunosorbent assay（ELISA） and RT-PCR assay.Results The concentration of 10, 20, 40 μg/mL of withaferin-A can inhibit proliferation of SK-MES-1 cells, the inhibition rate relat to the action concentrations; ELISA results indicated that the expression of E-cadherin was signifi- cantly lower than that of control group, and the expression of Vimentin was significantly increased compared with the control group at different concentrations 20 I^g/mL and 40 g/mL WFA, respectively.The results RT-PCR was that the concentration of 20 and 40 μg/mL WFA in group E-cadherin mRNA expression increased, Vimentin mRNA expression reduced,the difference was statistically significant（P 〈 0.05）.Conclusion WFA can inhibit the growth of lung squamous cancer cells and inhibit the ep- ithelial-interstitial conversion process,its occurrence relate to the raise of expression about E cadherin protein and gene,and the downgrade of expression Vimentin protein and gene.So we speculate that withaferin-A might inhibit lung squamous epithelial mes- enchymal changes of the cancer cells（EMT）,thus inhibiting tumor growth, providing new thought and way for clinical treatment of lung cancer.

关 键 词：醉茄素-A 肺鳞癌 上皮-间质转化 

分 类 号：R965[医药卫生—药理学]