亚硫酸氢钠穿心莲内酯和硫酸卡那霉素对HK-2细胞的毒性分析  

作　　者：胡中慧[1,2] 王全军[1] 李加恒[2] 王翔[2] 张红霞[2] 廖明阳[1] 

机构地区：[1]军事医学科学院毒物药物研究所·国家北京药物安全评价研究中心,北京100850 [2]中国人民解放军兰州军区联勤部药品仪器检验所,甘肃兰州830050

出　　处：《中国药业》2015年第B12期8-11,共4页China Pharmaceuticals

基　　金：重大新药创制科技重大专项,项目编号：2008ZX09305-003;北京市自然科学基金,项目编号：7092079.

摘　　要：目的比较亚硫酸氢钠穿心莲内酯和硫酸卡那霉素对HK-2细胞毒性作用的差异。方法通过倒置显微镜观察亚硫酸氢钠穿心莲内酯和硫酸卡那霉素作用后HK-2细胞形态的改变，应用噻唑蓝（M1Tr）比色法和流式细胞术法检测药物对HK-2细胞的形态、细胞周期、凋亡率、线粒体膜电位（MMP）和活性氧（ROS）的影响。结果亚硫酸氢钠穿心莲内酯对HK-2细胞作用后24h和48h的‰值为（19．12±3．55）和（n．56±3．881）玑；硫酸卡那霉素对HK-2细胞的作用后24h和48h的IC50值为（119．35±6．22）和（120．25±22．09）g／L，随着硫酸卡那霉素作用的时间增加，IC50值变化不显著。西药对HK-2细胞的形态、细胞周期、凋亡率、MMP和ROS的影响不同．联合用药对细胞的损伤作用更大。结论亚硫酸氢钠穿心莲酯和卡那霉素可能以不同的途径对HK-2细胞产生毒性，联合作用可加重对HK-2细胞毒性作用。Objective To compare the differences of drug combination concerning andrographolidi natrii bisulfis and kanamycin sulfate on HK-2 cytotoxicity.Methods The morphological changes of andrographolidi natrii bisulfis and kanamycin sulfate on HK- 2 cells were examined with contrast microscopy and growth inhibitory rate of HK-2 ceils were detected by MTT color/metric as- say and the cell-cycle arrest, apoptosis,mitochondrial membrane potential（MMP）and reactive oxygen species（ROS） were analyzed by flow cytometry.Results The IC50 of andrographolidi natrii bisulfis and kanamycin sulfate against HK-2 cells for 24 and 48 hours were （19.12±3.55）, （11.56±3.881 ）, （119.35±6.22）and （120.25±22.09）g/L respectively.The IC5o of kanamycin sulfate didn＇t change significantly with increase of time. And there were lots of differences in HK-2 cells morphology,cell cycle,MMP,and ROS respectively treated with andrographolidi natrii bisulfis and kanamycin sulfate and their combination induced greater injury on HK-2 cells than the two drugs alone.Conclusion Andrographolidi natrii bisulfis and kanamycin sulfate might act on HK-2 ceils in different pathways to produce toxic effects and their combined effect can exacerbate the more venenous on HK-2 cells.

关 键 词：亚硫酸氢钠穿心莲内酯 硫酸卡那霉素 人肾小管上皮细胞 线粒体膜电位 活性氧 

分 类 号：R965[医药卫生—药理学]