RP-HPLC法同时测定九味羌活丸中升麻素苷等5种有效成分的含量  

Simultaneous determination of five constituents' content in Jiuweiqianghuo pills by RP-HPLC

作　　者：李巧玲[1] 王冬梅[1] 苗秋艳[1] 余秋丽[1] 刘焕霞[1] 赵怀清[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016

出　　处：《沈阳药科大学学报》2016年第2期114-119,126,共7页Journal of Shenyang Pharmaceutical University

摘　　要：目的建立RP-HPLC法同时测定九味羌活丸中升麻素苷、升麻素、5-O-甲基维斯阿米醇苷、黄芩苷和汉黄芩苷5种有效成分的含量。方法采用Kromasil C_(18)(250 mm×4.6mm,5μm)色谱柱,柱温为35℃,流动相为乙腈(A)-体积分数0.05%的磷酸水溶液(B),梯度洗脱,流速为1.0 m L·min^(-1),检测波长为254 nm。结果升麻素苷、升麻素、5-O-甲基维斯阿米醇苷、黄芩苷和汉黄芩苷分别在质量浓度0.684~6.84、0.226~2.26、1.20~12.0、20.9~209和3.84~38.4 mg·L^(-1)内与峰面积呈良好的线性关系(r≥0.999 8,n=6),平均回收率分别为100.3%、100.2%、99.7%、101.0%和100.7%(RSD在1.0%~1.3%内,n=9)。结论该方法可用于九味羌活丸的质量控制。Objective To establish an RP-HPLC method for the determination of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, baicalin, wogonoside in Jiuweiqianghuo pills （ traditional Chinese medicines）. Methods The HPLC separation was achieved on a Kromasil C18 （ 250 mm × 4.6 mm,5 μm ）at 35 ℃. The mobile phase consisted of acetonitrile -0.05% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL·min^-1. The UV detection wavelength was set at 254 nm. Results The linear ranges were 0. 684 -6.84,0. 226 -2.26,1.20 - 12.0,20.9 -209 and 3.84 -38.4 mg·L^- 1 （ r≥ 0. 999 8, n = 6 ）, respectively. The average recoveriesof prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside, baicalin and wogonoside were i00. 3%, 100.2% ,99.7%, 101.0% and 100. 7% （RSD = 1.0% - 1.3%, n = 9 ）, respectively. Conclusions The method can be used for quality control of Jiuweiqianghuo pills.

关 键 词：九味羌活丸 升麻素苷 升麻素 5-O-甲基维斯阿米醇苷 黄芩苷 汉黄芩苷 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]