眼针八区八穴对脑缺血再灌注24h大鼠海马组织中MAPK信号传导通路的影响  

The Effects of MAPK Signal Transduction Pathway in Rat Hippocampus Pattern of Ischemia Reperfusion in 24h by Eye Acupuncture's Eight Districts 8 Points

作　　者：张威[1] 马贤德[2] 田维柱[1] 

机构地区：[1]辽宁中医药大学附属医院,沈阳110847 [2]辽宁中医药大学,沈阳110847

出　　处：《中国中医基础医学杂志》2016年第2期239-241,F0004,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：辽宁省科技厅博士科研启动基金项目(20131073);国家中医药管理局辽宁彭氏眼针学术流派传承工作室建设项目(LPGZS2012-09)

摘　　要：目的:观察八区八穴取穴眼针疗法对脑缺血再灌注损伤模型大鼠海马组织中p38MAPK、ERK1/2、JNK表达水平的影响,探讨八区八穴取穴眼针疗法治疗缺血性脑病的部分机制。方法:健康SPF级雄性SD大鼠40只,体质量(280±20)g。按体质量随机分组,分为空白对照组(8只)、假手术组(8只)和模型复制组(24只)。对模型复制组24只大鼠采用线栓法进行模型复制,模型评价后,筛选模型合格的大鼠进行亚组划分,分别为模型对照组、眼针对照组,即分为正常组、假手术组、模型组、眼针组4组。眼针组大鼠给予眼针干预,再灌注即刻以及之后的每8 h进行1次针刺治疗,至再灌注24 h取材进行相关指标检测。采用免疫组织化学法检测各组大鼠海马组织中P38MAPK、ERK1/2和JNK蛋白表达水平。结果:与空白对照组比较,模型对照组和眼针组大鼠海马组织中P38MAPK、ERK1/2、JNK蛋白表达水平均显著升高;与模型对照组比较,眼针组大鼠脑组织中P38MAPK、ERK1/2、JNK蛋白表达水平显著下降。结论:眼针可能通过抑制p38-MAPK信号传导通路而发挥其脑保护作用。To observe the influence of the P38MAPK, JNK, ERK1/2 protein expression in rats hippocampus cerebral ischemia reperfusion and to explore its mechanism. Methods：40 male SD rats were randomly divided into control group （8 rats）, sham operation group （8 rats） and model group （24rats）. The line plug method was used to replicate the model in model group. After evaluation, the qualified rats were divided into model group and eye acupuncture group. So the groups were divided as follows： control group, sham operation group, model group and eye acupuncture group. After cerebral ischemia and reperfusion, The rats were treated with acupuncture immediately, then treated with acupuncture at every 8h until to 24h of ischemia reperfusion respectively. Immunohistochemical method detect MAPK three mainly subfamily P38MAPK pathway, ERK1/2 and JNK protein expression. Results compared with the control group, the levels of P38MAPK, JNK, ERK1/2 protein expression were significantly increased in the model control group and the eye acupuncture group, compared with the model group, the P38MAPK, JNK, ERK1/2 protein expression in rats hippocampus were significantly decreased. Conclusion：eye acupuncture may play a protective role in the p38-MAPK signal transduction pathway.

关 键 词：八区八穴 脑缺血再灌注 眼针 P38MAPK JNK ERK. 

分 类 号：R246.8[医药卫生—针灸推拿学]