移植重编程星形胶质细胞对大鼠损伤脊髓恢复的影响  

Effect of transplantation of reprogmmed astrocytes on recovery of spinal cord injury in rats

作　　者：朱荣岚 陆晓诚 唐琳俊 黄宝胜 余万[4] 李帅 王希 李立新 

机构地区：[1]南京医科大学第一附属医院神经外科,江苏省210029 [2]苏州大学第一附属医院神经外科 [3]安徽省铜陵市立医院神经外科 [4]南京中医药大学附属中西医结合医院神经外科

出　　处：《江苏医药》2016年第4期379-382,F0002,共5页Jiangsu Medical Journal

基　　金：国家自然科学基金(81171147);江苏省重点人才资助项目(RC201156)

摘　　要：目的探讨移植经直接重编程后的星形胶质细胞对大鼠脊髓损伤功能恢复的影响。方法建立SD大鼠脊髓损伤模型48只,随机均分为四组,分别为移植转染了含有神经原素2(Ngn2)的星形胶质细胞组(A组)、移植转染了绿色荧光蛋白基因的星形胶质细胞组(B组)、移植星形胶质细胞组(C组)和移植PBS组(D组)。移植后4周,免疫荧光染色观察脊髓组织的神经元特异性核蛋白(NeuN)和微管相关蛋白2(MAP2)的表达;利用Basso Beattie and Bresnahan(BBB)评分系统对移植后的大鼠进行连续8周的BBB评分,观察其运动功能的恢复情况。结果连续观察8周后,A组的BBB评分高于其他三组(P<0.05)。A组的NeuN和MAP2表达均高于B组(P<0.05)。结论在大鼠脊髓损伤模型中,移植经直接重编程处理过的星形胶质细胞能提高受损脊髓组织的神经元数量,有助于损伤脊髓的运动功能恢复。Objective To study the effect of transplantation of reprogmmed astrocytes on the recovery of injured spinal cord in rats.Methods The rat model with spinal cord injury was established in 48 SD rats,which were equally randomized into four groups of A[transplantated the reprogmmed astrocytes transfected with neurogenin2（Ngn2）],B（transplantated the reprogmmed astrocytes transfected with green fluorescent protein）,C（transplantated with astrocytes）and D（transplantated with PBS）.The expressions of neuron-specific nuclear protein（NeuN）and microtubule-associated protein 2（MAP2）were detected by immunofluorescent staining four weeks after transplantation.The motor function was evaluated by Basso Beattie and Bresnahan（BBB）scoring for 8 weeks.Results The BBB score was higher in group A than that in groups of B,C and D（P〈0.05）.The expressions of NeuN and MAP2 were higher in group A than that in group B（P〈0.05）.Conclusion Transplantation of reprogmmed astrocytes to the injuried spinal cord may increase the number of neurons and benefit the recovery of motor function in rat models.

关 键 词：脊髓损伤 星形胶质细胞 神经原素 

分 类 号：R651[医药卫生—外科学]