HPLC同时测定四季三黄片中芦荟大黄素、黄芩苷、黄柏酮和西红花苷-Ⅰ的含量  

Simultaneous Determination of Aloe-emoidin, Baicalin, Obacunone and Crocin Ⅰ in Sijisanhuang Tablets by HPLC

作　　者：汪佳萍 柴军 吕林峰 

机构地区：[1]绍兴市食品药品检验中心,浙江绍兴312071

出　　处：《中国现代应用药学》2016年第2期199-203,共5页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的采用HPLC同时测定四季三黄片中芦荟大黄素、黄芩苷、黄柏酮和西红花苷-Ⅰ的含量。方法采用Welch Topsil-C18色谱柱(4.6 mm×250 mm,4μm);流动相:甲醇-0.2%磷酸水溶液;梯度洗脱;检测波长分别为254 nm(芦荟大黄素、黄芩苷、黄柏酮)和440 nm(西红花苷-Ⅰ);流速:1.0 m L·min^(-1);柱温:30℃。结果芦荟大黄素、黄芩苷、黄柏酮和西红花苷-Ⅰ线性范围分别为0.079 10~1.582μg·m L^(-1)(r=0.999 5),0.167 5~3.350μg·m L^(-1)(r=0.999 8),0.097 92~1.958μg·m L^(-1)(r=0.999 7)和0.033 57~0.671 5μg·m L^(-1)(r=0.999 4),平均加样回收率分别为95.9%(RSD=0.7%),97.5%(RSD=0.8),96.4%(RSD=1.0%),95.5%(RSD=1.3%)。结论该方法简便、准确、专属性强、重复性好,可用于四季三黄片中芦荟大黄素、黄芩苷、黄柏酮和西红花苷-Ⅰ的定量分析。OBJECTIVE To establish an HPLC method for the simultaneous determination of aloe-emoidin, baicalin, obacunone and crocin Ⅰ in Sijisanhuang tablets. METHODS A Welch Topsil-C18 column（4.6 mm×250 mm, 4 μm） was used with the mobile phase of methanol-0.2% phosphate solution at a flow rate of 1.0 mL·min^-1, the column temperature was 30 ℃ and the detection wavelength was 254 nm for aloe-emoidin, baicalin, obacunone and 440 nm for crocin I. RESULTS The calibration curves of aloe-emoidin, baicalin, obacunone and crocin Ⅰ were linear over the ranges of 0.079 10-1.582 μg·mL^-1 （r=0.999 5）, 0.167 5-3.350 μg·mL^-1（r=0.999 8）, 0.097 92-1.958 μg·mL^-1（r=0.999 7）, 0.033 57-0.671 5 μg·mL^-1（r=0.999 4）, respectively, and the average recoveries were 95.9%（RSD=0.7%）, 97.5%（RSD=0.8）, 96.4%（RSD=1.0%）, 95.5%（RSD=1.3%）, respectively. CONCLUSION The method is simple, accurate, specific and repeatable, which can be used for quantitative analysis of aloe-emoidin, baicalin, obacunone and crocin I in Sijisanhuang tablets.

关 键 词：四季三黄片 芦荟大黄素 黄芩苷 黄柏酮 西红花苷-Ⅰ 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学]