Beagle犬血浆中尼莫地平的UPLC-MS/MS法测定  

Determination of Nimodipine in Beagle Dog Plasma by UPLC-MS/MS

作　　者：向志雄[1] 刘文丽[1] 

机构地区：[1]上海医药集团股份有限公司中央研究院,上海201203

出　　处：《中国医药工业杂志》2016年第3期316-319,共4页Chinese Journal of Pharmaceuticals

摘　　要：建立了超高效液相色谱-串联质谱法测定Beagle犬血浆中的尼莫地平。以尼群地平为内标,使用Waters Cortecs C18色谱柱,采用电喷雾电离源（ESI）,多反应监测（MRM）模式,正离子检测。尼莫地平在0.1~300 ng/ml范围内线性关系良好。日内、日间RSD均小于15%。同时测定了Beagle犬口服尼莫地平片后的血药浓度,主要药动学参数分别为：tmax（0.7±0.5）h,t1/2（5.5±3.9）h,cmax（322.3±181.0）ng/ml,AUC0→t（833.3±412.7）ng·h·ml^-1,AUC0→∞（870.6±448.9）ng·h·ml^-1,MRT（5.2±2.3）h。An UPLC-MS/MS method was established for the determination of nimodipine in Beagle dog plasma. Nitrendipine was chosen as the internal standard. A Waters Cortecs C18 column was used, with the application of electrospray ion source （ESI） in the positive ion mode, combined with multiple reaction monitoring （MRM） mode. It was linear for nimodipine in the range of 0.1--300 ng/ml. The intra- and inter-day RSDs were less than 15%. The nimodipine concentration in plasma of Beagle dogs after oral administration of nimodipine tablets was determined. The main pharmacokinetic parameters were as follows： tmax（0.7±0.5）h,t1/2（5.5±3.9）h,cmax（322.3±181.0）ng/ml,AUC0→t（833.3±412.7）ng·h·ml^-1,AUC0→∞（870.6±448.9）ng·h·ml^-1,MRT（5.2±2.3）h.

关 键 词：尼莫地平 超高效液相色谱-串联质谱 测定 

分 类 号：TQ463.54[化学工程—制药化工]