Src羧基末端激酶结合蛋白过表达及棕榈酰化对裸鼠T系白血病Jurkat细胞增殖作用影响的研究  

Effect of over expression of C-terminal Src kinase binding protein(Cbp) and Cbp palmitoylation to proliferation of T-leukemia Jurkat cell in nude mice

作　　者：马宇[1] 高美华[1] 张敏锐[1] 张蓓[1] 王冰[1] 杨丽华[1] 

机构地区：[1]青岛大学医学院免疫学教研室,青岛266071

出　　处：《中国免疫学杂志》2016年第1期51-55,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金项目(No.81273206)

摘　　要：目的:建立Src羧基末端激酶结合蛋白(Cbp)过表达及棕榈酰化的T系白血病Jurkat细胞裸鼠模型,研究Cbp过表达及棕榈酰化对Jurkat细胞增殖作用的影响。方法:利用neg-EGFP、Cbp-EGFP、Cbp-m-EGFP融合蛋白慢病毒载体转染的Jurkat细胞构建动物模型,24只5周龄雌性BALB/c-nu小鼠随机分为空白对照组、空病毒对照组、Cbp过表达组和Cbp棕榈酰化位点突变组,每组6只。接种前和接种后0、1、2、3、4、5周裸鼠称重,取外周血计数白细胞总数。利用病毒本身携带的EGFP绿色荧光蛋白在激光共聚焦显微镜下观察Jurkat细胞在外周血中的增殖情况;HE染色观察肝脏的病理变化;流式细胞仪检测Jurkat细胞在小鼠骨髓和外周血中的增殖水平。结果:Cbp过表达组小鼠体重低于空白对照组,高于空病毒对照组和Cbp棕榈酰化位点突变组,Cbp棕榈酰化位点突变组小鼠体重低于空白对照组、空病毒对照组和Cbp过表达组。Cbp过表达组小鼠外周血白细胞总数和肝脏、骨髓、外周血中Jurkat细胞增殖水平高于空白对照组、低于空病毒对照组和Cbp棕榈酰化位点突变组。Cbp棕榈酰化位点突变组小鼠外周血白细胞总数和肝脏、骨髓、外周血中Jurkat细胞增殖水平高于空白对照组、空病毒对照组和Cbp过表达组。结论:成功建立了Cbp过表达及棕榈酰化的T系白血病Jurkat细胞动物模型,Cbp过表达可抑制Jurkat细胞增殖,Cbp棕榈酰化位点突变可促进Jurkat细胞增殖。Objective：To establish T lineage leukemia Jurkat cell mice model of over expression of C-terminal Src kinase binding protein（ Cbp ） and Cbp palmitoylation and to research the effect of Cbp and Cbp palmitoylation to proliferation of Jurkat cell. Methods： Virus transfected cell of neg-EGFP, Cbp-EGFP and Cbp-m-EGFP were used in mice model. 24 female BALB/c-nu mice were randomly divided into blank control group, empty virus control group, over expression of CBP group and Cbp palmitoylation group, 6 mice in each group. The nude mice were weighed in 0,1,2,3,4,5 weeks. The amount of white blood cell in peripheral blood were counted in 0, 1,2, 3,4,5 weeks. The proliferation of Jurkat cell in peripheral blood of mice were observed by laser confocal microscope. The pathological changes of liver were observed using HE staining. The proliferation of Jurkat cell in the bone marrow and peripheral blood of mice were detected with flow cytometry. Results： The weight of mice in over expression of Cbp group was less than that in blank control group, but higher than that in empty virus control group and Cbp palmitoylation group. The weight of mice in Cbp palmitoylation group was less than that in blank control group ,empty virus control group and over expression of Cbp group. The amount of white blood cell in peripheral blood and proliferation of Jurkat cell in liver, bone marrow and peripheral blood of mice in over expression of Cbp group was higher than that in blank control group, but less than that in empty virus control group and Cbp palmitoylation group. The amount of white blood cell in peripheral blood and proliferation of Jurkat cell in liver, bone marrow and peripheral blood of mice in Cbp palmitoylation group was higher than that in blank control group, empty virus control group and over ex- pression of Cbp group. Conclusion： Over expression of Cbp and Cbp palmitoylation in T lineage leukemia.Jurkat cell mice model was established. Over expression of Cbp has inhibitory effect on the proliferation of Jurkat ce

关 键 词：Src羧基末端激酶结合蛋白(Cbp) T系白血病 JURKAT细胞 裸鼠模型 

分 类 号：R392.4[医药卫生—免疫学]