松江鲈线粒体DNA控制区结构和遗传多样性分析  

ANALYSIS OF THE STRUCTURE OF MITOCHONDRIAL DNA CONTROL REGION AND THE GENETIC DIVERSITY OF TRACHIDERMUS FASCIATUS IN DIFFERENT POPULATIONS

作　　者：赵林林[1] 毕潇潇[1] 宋林[2] 高天翔[1] 

机构地区：[1]中国海洋大学海洋生物多样性与进化研究所,青岛266003 [2]辽东学院,丹东118001

出　　处：《水生生物学报》2016年第1期35-41,共7页Acta Hydrobiologica Sinica

基　　金：海洋公益性行业科研专项项目(201305043;201405010)资助~~

摘　　要：为探讨松江鲈(Trachidermus fasciatus Heckel)线粒体控制区特征及其群体遗传结构,研究测定分析了中国和日本沿海共8个群体的线粒体控制区序列,分析了其结构特征,识别出终止序列区(ETAS)、中央保守区(CD)和保守序列区(CSB)的特征序列。遗传多样性分析结果显示:69个松江鲈个体共检测到47个单倍型,呈现出核苷酸多样性(0.0079)较低和单倍型多样性(0.978)较高的特点。单倍型邻接关系树和单倍型网络关系图均显示松江鲈分为中国和日本两大世系。遗传分化系数(F_(st))和分子方差分析(AMOVA)结果表明,松江鲈中国群体和日本群体之间存在的遗传差异较显著,中国沿海各群体之间亦存在着一定程度的遗传差异,该分化主要由历史环境变动、当代环境因素和自身生态习性等原因造成。Roughskin sculpin, also known as Trachidermusfasciatus, is a catadromous fish that live in the seawater along the coast of the northwest Pacific Ocean. The fish population size has been largely reduced in recent years due to the deterioration of the habitats, water pollution and the construction of dams. To protect the fish resource and to improve the fishery management, we need to better understand the genetic structure of the fish population. In this study, we collected 69 individuals from 8 sampling sites near the coast of China and Japan, including Hangzhouwan （HZ）, Wendeng （WD）, Rongcheng （RC）, Dongying （DY）, Qinhuangdao （QH）, Dalian （DL）, Dandong （DD） and Ariake Sea （AS）. The mitochondrial control region of T. fasciatus was amplified with polymerase chain reaction （PCR） technology and was sequenced with the forward and reverse primers by the automatic sequencer. We found that the 8 populations exhibited high haplotype diversity （0.978） and low nucleotide diversity （0.0079）. Our analysis of the control region revealed the terminal associated sequence, the central conserved domains and the conserved sequence block domains. Moreover, the analysis ofNJ phylogenetic trees and mediannetworks demonstrated that these populations could be divided into the Chinese lineage and the Japanese lineage. Finally, both the genetic differentiation index （Fst） and the analysis of molecular variance （AMOVA） indicated a significant genetic differentiation between the Japanese population and the Chinese populations, as well as a moderate differentiation within the Chinese populations.

关 键 词：松江鲈 控制区结构 遗传多样性 遗传分化 

分 类 号：Q346.5[生物学—遗传学]