基于人胚胎干细胞健康安全评价体系的构建  

Development of human embryonic stem cell platforms for human health-safety evaluation

作　　者：俞光岩[1] 曹彤 邹晓晖[3] 张学慧[4] 傅歆 彭双清[6] 邓旭亮[7] 李盛林[1] 刘鹤[8] 肖苒 欧阳宏伟[3] 彭晖[9] 陈晓[3] 赵增明[6] 王晓颖[1] 方海琴[6] 路璐[1] 任玉兰[8] 徐明明[7] 

机构地区：[1]北京大学口腔医学院.口腔医院口腔颌面外科,北京100081 [2]Faculty of Dentistry Research Laboratories,National University of Singapore [3]浙江大学干细胞和组织工程中心,杭州310058 [4]北京大学口腔医学院.口腔医院口腔材料研究室,北京100081 [5]中国医学科学院整形外科医院研究中心,北京100144 [6]军事医学科学院疾病预防控制研究所毒理学评价研究中心,北京100071 [7]北京大学口腔医学院.口腔医院特诊科,北京100081 [8]北京大学口腔医学院.口腔医院儿童口腔科,北京100081 [9]北京大学口腔医学院.口腔医院第三门诊部,北京100081

出　　处：《北京大学学报（医学版）》2016年第1期1-4,共4页Journal of Peking University:Health Sciences

基　　金：科技部国际科技合作项目(2011DFA32190)资助~~

摘　　要：食品、药品、医疗器械和材料、生物制剂以及环境的质量和健康安全是政府和民众十分关注的热点问题。目前国际标准的健康安全评价检测方法以微生物、动物及其细胞作为载体,不能准确反映人类的生物学特征,预测人体心、肝等靶器官毒性及发育毒性的准确率偏低,在技术层面严重制约了人类健康安全的评价和监控。如何建立准确反映人类生物学特征的健康安全评价体系是关系国民健康安全的重大科学问题^（[1]）。The human embryonic stem cells （hESCs） serve as a self-renewable, genetically-healthy, pluripotent and single source of all body cells, tissues and organs. Therefore, it is considered as the good standard for all human stem cells by US, Europe and international authorities. In this study, the standard and healthy human mesenchymal progenitors, ligament tissues, cardiomyocytes, keratinocytes, primary neurons, fibroblasts, and salivary serous cells were differentiated from hESCs. The human cellular health-safety of NaF, retinoic acid, 5-fluorouracil, dexamethasone, penicillin G, adriamycin, lead ace- tate PbAc, bisphenol A-biglycidyl methacrylate （Bis-GMA） were evaluated selectively on the standar- dized platforms of hESCs, hESCs-derived cardiomyocytes, keratinocytes, primary neurons, and fibro- blasts. The evaluations were compared with those on the currently most adopted cellular platforms. Parti- cularly, the sensitivity difference of PM2.5 toxicity on standardized and healthy hESCs derived fibroblasts, currently adopted immortalized human bronchial epithelial ceils Beas-2B and human umbilical vein endo- thelial cells （HUVECs） were evaluated. The results showed that the standardized hESCs cellular plat- forms provided more sensitivity and accuracy for human cellular health-safety evaluation.

关 键 词：胚胎干细胞 毒性试验 细胞分化 体外研究 人类 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]