腹腔间隔室综合征大鼠血清D-乳酸及二胺氧化酶的变化及意义  

The changes and significance of serum D-lactate and DAO in rats with abdominal compartment syndrome

作　　者：刘童蕾 曾玉剑[2] 田衍[2] 舒若[2] 孙亮[2] 王沛云[1] 肖博凯 施承民 郭姝婧 张同[2] 罗华友[2] 

机构地区：[1]昆明医科大学,昆明650032 [2]昆明医科大学第一附属医院胃肠与疝外科,昆明650032

出　　处：《国际外科学杂志》2016年第1期23-25,F0003,共4页International Journal of Surgery

基　　金：2014年度云南省医疗卫生单位内设研究机构科研项目(2014NS124) Program of Yunnan Health Department

摘　　要：目的 研究腹腔间隔室综合征（ACS）大鼠血清D-乳酸、二胺氧化酶（DAO）的变化及意义.方法 成年SD大鼠40只,按配对比较法随机分为对照组和实验组（各20只）,实验组予建立ACS模型,对照组予假手术,建模成功后取大鼠血清检测D-乳酸及DAO含量（ELISA法）;取小肠组织观察病理形态改变.结果 实验组大鼠小肠黏膜和黏膜下水肿、糜烂,部分上皮细胞坏死、脱落,其血清D-乳酸及DAO含量分别为（4.46±3.80） μg/mL、（1.24±2.09） U/L,对照组血清D-乳酸及DAO含量分别为（2.08±1.08） μg/mL、（0.11±0.11） U/L,二者比较差异有统计学意义（P＜0.05）.结论 ACS大鼠血清D-乳酸及DAO含量与肠屏障功能损伤程度相关,D-乳酸及DAO可作为ACS病情的主要监测指标之一.Objective To investigate the level of D-lactate and DAO in serum and explore significance in the rats with ACS.Methods Forty adult SD rats were assigned randomly to sham operation group and experimental group.Rats in experimental group were established ACS model.After sacrificed,the level of DAO and D-lactate in serum were measured by using ELISA,the morphologic changes were observed with microscope.Results The small intestine mucosa and submucosal tissue were oedematous or anabrotic,and the part of epithelial cell were necrotic in the rats with ACS.The expressions of D-lactate and DAO in the experimental group were significantly higher than those in the control group [（4.46 ± 3.80） vs （2.08 ± 1.08） μg/mL and （1.24 ± 2.09） vs （0.11 ± 0.1 1） U/L （P 〈 0.05）].Conclusions The level of serum D-lactate and DAO is related to the degree of damage of the gut barrier function in the rats with ACS,and they can be helpful to monitor the activity of ACS.

关 键 词：腹腔间隔室综合征 D-乳酸 胺氧化酶 模型 动物 

分 类 号：R656[医药卫生—外科学]