静宁复方有效成分大鼠在体肠吸收研究  

In vivo Absorptive Dynamics of Active Ingredients of JingNing Compound Prescribe in Intestine of Rats

作　　者：杨春静[1] 倪健[1] 曹飒丽[1] 胡伊力格其[2] 薛丹[1] 夏振文[1] 赵阳[1] 尹兴斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]内蒙古民族大学附属医院,内蒙古通辽028000

出　　处：《现代中药研究与实践》2016年第1期26-30,共5页Research and Practice on Chinese Medicines

基　　金：北京市"十病十药"研发项目(Z141100002214020);国家自然科学基金面上项目(81173563);北京中医药大学协同创新建设计划(2013-XTCX-03)

摘　　要：目的研究静宁复方中五味子醇甲、醇乙、甲素、乙素四种木脂素类成份在大鼠小肠内的吸收动力学特性。方法选用在体大鼠肠循环法,考察静宁复方在大鼠小肠的吸收受浓度影响情况,计算四种成分的的吸收速率常数（Ka）、吸收半衰期（T1/2）、表观渗透系数（Papp）、单位时间吸收率（P）。结果除五味子乙素（2.3~9.2μg/ml）外,五味子醇甲（8.5~34μg/ml）、醇乙（1.8~7.0μg/ml）、甲素（1.4~5.4μg/ml）的四个参数值不随质量浓度的变化而变化。结论静宁复方提取物中五味子醇甲、醇乙、甲素在大鼠小肠的吸收不同浓度的Ka值均无显著性差异,且吸收量与浓度不成正比,不符合Ficks方程,研究表明,三种活性成分不仅是被动扩散,也可能有载体介质的转运方式。乙素可能为主动转运。Objective To research rat enteric in-take mechanism of four active lignans, Schisandrol A, Schisandrol B, Schisandrin A, Schisandrin B. Methods Establishd enteric perfusion model in suit and used HPLC to determinate in-take difference of the four active ingredients in different concentrations. Results The result of in situ enteric perfusion display that three active compounds of Schisandrol A, Schisandrol B, Schisandrin A, the four parameter values in the intestinal absorption were not notly different within the concentration range （P 〉 0.05）, Schisandrin B, the four parameter values in the intestinal absorption were significantly different within the concentration range in the study （P 〈 0.05）. Conclusion Schisandrol A, Schisandrol B, Schisandrin A do not complies with the ticks equation and the absorptive mechanism in intestine may be carrier-mediated transport. Schisandrin B is likely to be active transport.

关 键 词：五味子醇甲 五味子醇乙 五味子甲素 五味子乙素 肠吸收 

分 类 号：R285.5[医药卫生—中药学]