HPLC法同时测定桑菊清肺合剂中三种成分的含量  

Simultaneous Determination of Three Components in Sangju Qingfei Mixture by HPLC

作　　者：陈国宝[1] 宋桂萍[1] 张祥霞[1] 柳佳[1] 

机构地区：[1]南京中医药大学江阴附属医院,江苏江阴214400

出　　处：《现代中药研究与实践》2016年第1期51-54,共4页Research and Practice on Chinese Medicines

基　　金：2013年度江阴市中医院青年中医科学基金资助项目:清肺止咳合剂制剂成型工艺研究(JY 130901)

摘　　要：目的建立桑菊清肺合剂中指标性成分木犀草苷、绿原酸、连翘苷的含量测定方法。方法采用Venusil XBP C18色谱柱（250 mm×4.6 mm,5μm）,流动相采用甲醇（A）-0.4%乙酸（B）,梯度洗脱程序为：0~10 min,A：22~40%;10~45 min,A：40%,流速为1.0 ml/min,检测波长为277 nm,柱温35℃,进样量为10μl。结果木犀草苷在0.072 0~0.252 0 mg/ml范围内线性关系良好（r=0.998 9）,回收率为102.8%（RSD=1.59%,n=5）;绿原酸在0.134 8~0.968 8 mg/ml范围内线性关系良好（r=0.999 5）,回收率为100.6%（RSD=0.25%,n=5）;连翘苷在0.153 2~0.536 2 mg/ml范围内线性关系良好（r=0.998 5）,回收率为95.56%（RSD=0.43%,n=5）,本研究建立的方法精密度、稳定性和加样回收率均符合含量测定要求。结论本法简单易行,结果准确可靠,可有效地用于桑菊清肺合剂的质量控制。Objective To establish the HPLC method for determining phillyrin, galuteolin, chlorogenic acid in Sangju Qingfei Mixture. Methods HPLC assay was carried out on a column ofVenusil XBP （250 mm×4.6 mm, 5 μm）. The mobile phase were methanol （A） and 0.4% acetic acid solution （B）. The elution program was 0 - 10 min, A： 22 - 40%; 10 - 45 min, A： 40%. The flow rate was 1.0 ml/min. The injection volume was 10 μl. The detection wavelength was set at 277 nm and the column temperature was 35℃. Results The galuteolin showed good linear correlations （r = 0.998 9） in the range of 0.072 0 - 0.252 0 mg/ml. The recovery was 102.8% （RSD = 1.59%, n -- 5）. The chlorogenic acid showed good linear correlations （r = 0.999 5） in the range of 0.134 8 - 0.968 8 mg/ml. The recovery was 100.6% （RSD = 0.25%, n = 5）. The phillyrin showed good linear correlations （r = 0. 998 5） in the range of 0.153 2 - 0.536 2 mg/ml. The recovery was 95.56% （RSD = 0.43%, n = 5）. The precision, accuracy and recovery tests meet the determination requirements. Conclusion The method is simple, reliable, accurate and very suitable for the quality control of Sangju Qingfei Mixture.

关 键 词：桑菊清肺合剂 木犀草苷 连翘苷 绿原酸 含量测定 

分 类 号：R927.2[医药卫生—药学]