普伐他汀对人胰腺癌细胞SW1990增殖和凋亡的影响及其机制研究  

Effects of Pravastatin on the Proliferation and Apoptosis of Human Panceatic Cancer Cell SW1990 and Its Mechanisms

作　　者：张敏[1] 孔雁[2] 姜达[2] 程敏菊[1] 郭治[1] 

机构地区：[1]邢台市人民医院肿瘤内科,河北邢台054001 [2]河北医科大学第四医院肿瘤内科,河北石家庄050000

出　　处：《肿瘤药学》2016年第1期49-53,共5页Anti-Tumor Pharmacy

摘　　要：目的探讨普伐他汀(Pravastatin)对人胰腺癌细胞SW1990增殖和凋亡的影响及其可能机制。方法体外培养人胰腺癌细胞SW1990,给予不同浓度(0,5,10,20μmol·L-1)普伐他汀处理,采用四甲基偶氮唑蓝(MTT)比色法检测细胞增殖情况,流式细胞术测定细胞凋亡情况及p21Ras、Bcl-2、Bax蛋白表达,RT-PCR法检测Bcl-2、Bax m RNA水平。结果普伐他汀处理SW1990细胞后,细胞增殖受抑而凋亡增加,p21Ras、Bcl-2的蛋白表达降低而Bax的表达增高,Bcl-2 m RNA水平降低而Bax m RNA水平增高。结论普伐他汀可抑制胰腺癌细胞SW1990增殖并诱导其凋亡,其诱导凋亡机制可能是下调p21Ras、Bcl-2的表达和上调Bax的表达。Objective To investigate the effects of pravastatin on the proliferation and apoptosis of human pancreatic cancer cells SW1990 and its possible mechanisms. Methods SW1990 cells were cultured in vitro and treated with different concentrations （0, 5, 10, 20μmol.L-1） of pravastatin. Effects of pravastatin on the proliferation of SW1990 cells were measured by MTI＇ colorimetric method. The apoptosis of SW1990 cells and expression of p21Ras, Bcl-2 and Bax were examined with flow eytometry. The analysis of mRNA of Bcl-2 and Bax was shown by RT-PCR. Results After SW1990 cells were treated with pravastatin, the cell proliferation was inhib- ited and the cell apoptosis was increased. The protein expression of p21Ras and Bc]-2 decreased while the protein expression of Bax increased. The mRNA level of Bcl-2 decreased but that of Bax increased. Conclusions Pravastatin could inhibit the proliferation of SW1990 cell and induced its apoptosis. Its mechanism may involve the down-regulation of p21Ras, Bcl-2 and up-regulation of Bax.

关 键 词：普伐他汀 胰腺癌 增殖 凋亡 SW1990 

分 类 号：R96[医药卫生—药理学]