自噬调控剂对小鼠体内弓形虫增殖的影响  

Influence of Autophagy Regulation Agent on the Proliferation of Toxoplasma Gondii in Mice

作　　者：张琼[1] 张婧[2] 高劲松[1] 李万峰[1] 汪学龙[2] 

机构地区：[1]长沙医学院病原生物学教研室,长沙410219 [2]安徽医科大学病原生物学教研室

出　　处：《数理医药学杂志》2016年第3期319-321,共3页Journal of Mathematical Medicine

基　　金：湖南省教育厅科研基金资助项目(编号:10C0448);安徽省教育厅高校省级自然科学研究项目(编号:KJ2013A149)

摘　　要：目的:研究自噬调控剂对小鼠体内弓形虫增殖的影响。方法:建立弓形虫感染的小鼠模型,分别使用自噬抑制剂巴弗洛霉A1(bafilomycin A1)和自噬诱导剂氯化锂对弓形虫感染小鼠模型进行干预,应用荧光定量PCR检测不同实验条件下弓形虫数量。结果:实验结果表明巴弗洛霉素A1剂量与小鼠体内弓形虫数量呈负相关,而诱导剂氯化锂与小鼠体内弓形虫数量呈正相关。结论:调控宿主细胞自噬可调控体内弓形虫增殖。Objective: To study the influence of autophagy regulation agent on the proliferation of toxoplasma gondii in mice. Methods: Using autophagy inhibitors bafilomycin A1 and autophagy iinducing agent lithium chloride respectively to intervene toxoplasma gondii infected mice. Fluorescence quantitative PCR was used to detect proliferation of Toxoplasma gondii under different experimental conditions. Results: Quantitative PCR results showed that bafilomycin A1 dose and the number of toxoplasma gondii in mice was negative correlated,while inducing agent lithium chloride and the number of toxoplasma gondii in mice was positive correlated. Conclusion: The regulation on autophagy of host cells in mice could regulate the proliferation and replication of toxoplasma gondii.

关 键 词：弓形虫 自噬调控剂 荧光定量PCR 增殖 小鼠 

分 类 号：Q95-3[生物学—动物学]