5F-Na注射液对人肝癌MHCC97L细胞毒性及凋亡的影响  

Effects of 5F-Na on cytotoxicity and apoptosis on human liver cancer MHCC97L cells

作　　者：符伟玉[1] 蓝柳波 覃燕梅[1] 吴科锋[2] 马晓鹂[3] 

机构地区：[1]广东医学院生物化学与分子生物学研究所,广东湛江524023 [2]广东天然药物研究与开发重点实验室,广东湛江524023 [3]广东医学院附属医院药学部,广东湛江524023

出　　处：《药学研究》2016年第1期10-12,21,共4页Journal of Pharmaceutical Research

基　　金：广东省自然科学基金资助项目(No.S2012010008145);广东省科技计划项目(No.2011B031800343);湛江市财政资金科技专项竞争性分配项目(No.2013A01008)

摘　　要：目的探讨5F-Na对低转移型人肝癌MHCC97L细胞的抗癌效应。方法不同浓度5F-Na注射液(20、40、80μg·m L^(-1))处理MHCC97L细胞,MTT法检测细胞生长抑制率;Annexin V-EGFP染色及半胱氨酸蛋白酶-3活性检测细胞凋亡;流式细胞术检测细胞周期。结果 5F-Na注射液对MHCC97L细胞增殖抑制作用呈明显的时间、剂量依赖关系;不同浓度5F-Na注射液作用细胞24 h后,细胞凋亡比例较对照组明显升高(P<0.05);并可使细胞阻滞于G_2期。结论 5F-Na注射液抑制MHCC97L增殖与阻滞细胞于G_2期,促进凋亡有关。Objective To investigate the anticancer effect of 5F,a compound isolated from Pteris semipinnata L.( Ps L),on human liver cancer MHCC97 L cells. Methods MHCC97 L cells were treated with 5F- Na( 20,40,80μg·m L^(-1)) for different periods. Cytotoxicity was examined using MTT method. Apoptosis was examined using Annexin V- EGFP staining. Flow cytometry was used to detect the intracellular ROS level and cell cycle. Results 5F- Na inhibited the proliferation of MHCC97 L cells in a concentration and time- dependent manner. The apoptosis induced by 5F- Na was confirmed by positive staining of Annexin V- EGFP in MHCC97 L cells( P < 0. 05). 5F- Na arrested the cells in the G_2 phase and increased in the intracellular ROS level. Conclusion 5F- Na can inhibit the proliferation of MHCC97 L cells,at least partially,by arresting the cells in G_2 phase and increased the intracellular ROS level.

关 键 词：半边旗 5F-Na 肝癌 细胞凋亡 

分 类 号：R965[医药卫生—药理学]