CCK-8法检测药物影响肿瘤细胞增殖的优化研究  

Optimization study on the detection of tumor cell proliferation with CCK-8 method

作　　者：张会鲜[1] 何琪杨[1] 

机构地区：[1]中国医学科学院北京协和医学院医药生物技术研究所,北京100050

出　　处：《药学研究》2016年第2期63-66,共4页Journal of Pharmaceutical Research

基　　金：国家自然科学基金课题(No.81273553);北京协和医学院2015年研究生创新基金(No.2015-1007-18)

摘　　要：目的通过优化CCK-8的实验条件,提高检测药物影响肿瘤细胞增殖的敏感性和可靠性。方法以人肺癌A549细胞和白血病Molt-4细胞为研究对象,CCK-8法和MTT法分别检测细胞增殖的变化,并与Coulter细胞计数法和克隆形成率法进行比较。结果在相同的细胞数下,CCK-8法的检测灵敏度明显高于MTT法。以细胞密度1 000个/孔接种,抗肿瘤药物多柔比星处理96 h后,CCK-8法检测到的变化与克隆形成率法相当。分析Molt-4悬浮细胞发现:CCK-8法与Coulter细胞计数法的检测灵敏度无明显差异(P>0.05)。结论优化实验条件后,CCK-8法是一种灵敏度高、可靠性良好的细胞增殖检测方法。Objective To optimize the experiment condition and increase the reliability and sensibility of CCK- 8method in detecting the proliferation of tumor cells by drugs. Methods The proliferations of A549 and Molt- 4 cells were detected by CCK- 8 and MTT method,respectively. Meanwhile the sensitivity and accuracy were compared to cloning formation assay and Coulter cytometer. Results The sensitivity of CCK- 8 assay was significantly higher than that of MTT assay at the same cell density. The reliability of CCK- 8 was equivalent to the efficiency of clone formation assay when the cell density was seeded at 1 000 per well and treated with doxorubicin for 96 h. There were no significant differences in sensitivity and reliability of CCK- 8 and Coulter cytometer assay on suspension cells( P > 0. 05). Conclusion CCK- 8 is a kind of good assay for cell viability with a higher sensitivity and reliability during improved experiment conditions.

关 键 词：CCK-8法 细胞增殖 药效 多柔比星 

分 类 号：R965.2[医药卫生—药理学]