HPLC法测定肺胀颗粒中橙皮苷和甘草苷的含量  

Simultaneous determination of hesperidin and liquiritin content in Lung-distension granules by HPLC

作　　者：唐菱[1] 侯晓军[1] 王杰[2] 刘梅[1] 

机构地区：[1]重庆市中医院药剂科,重庆400021 [2]重庆市中医院呼吸科,重庆400021

出　　处：《应用化工》2016年第2期370-372,共3页Applied Chemical Industry

基　　金：重庆市卫生和计生委中医药科技项目(ZY201402030)

摘　　要：建立同时测定肺胀颗粒中橙皮苷和甘草苷含量的方法。采用HPLC-UV法,Diamonsil C_（18）柱（4.6 mm×150 mm,5μm）,流动相为0.2%磷酸水溶液-乙腈,梯度洗脱,检测波长为283 nm,柱温为30℃,流速为1 mL/min。橙皮苷和甘草苷分别在3.812~114.36μg/mL和1.874~56.22μg/mL范围内线性关系良好,平均回收率分别为100.2%,98.87%,RSD分别为1.45%,1.15%。该法简便、准确、灵敏、重现性好,可用于肺胀颗粒中橙皮苷和甘草苷含量的同时测定。To establish a method for simultaneous determination of hesperidin and liquiritin content in Lung-distension granules. A HPLC-UV method was developed. The separation was performed on a Diamonsil C18 column （4.6 mm× 150 mm,5 μm）. The mobile phase consisted of 0.2% phosphoric acidacetonitrile with gradient elution at the flow rate of 1.0 mlMmin. The detection wavelength was 283 nm. The column temperature was 30 ℃. The calibration curves of hesperidin and liquiritin showed good linearity in the ranges of 3.812 - 114.36 μg/mL and 1. 874 - 56.22 μg/mL. The average recoveries were 100.2% and 98.87% with RSD of 1.45% and 1.15%. The method is simple, accurate, sensitive and reproducible for simultaneous determination of hesperidin and liquiritin content in Lung-distension granules.

关 键 词：HPLC 肺胀颗粒 橙皮苷 甘草苷 含量测定 

分 类 号：TQ460[化学工程—制药化工] R917[医药卫生—药物分析学]