RGC-32在大鼠主动脉损伤后的表达  

Expression of RGC- 32 in Rat Injured Aorta

作　　者：黎思毅[1] 彭红娟[2] 

机构地区：[1]广东医学院附属医院血管甲状腺乳腺外科,广东湛江524001 [2]广东医学院附属医院眼科,广东湛江524001

出　　处：《吉林医学》2016年第1期5-8,共4页Jilin Medical Journal

基　　金：2011年广东省湛江市广东医学院青年基金面上项目[项目编号:XK1220]

摘　　要：目的:对RGC-32在大鼠主动脉损伤后的表达情况作初步研究,为血管术后再狭窄防治提供新思路。方法:选用雄性SD大鼠16只,大鼠随机分为两组。手术组:8只大鼠行球囊损伤大鼠主动脉术,术后28 d处死。对照组:8只大鼠,除不插入2F-Fogarty球囊导管进行损伤外,其他处理措施均与手术组相同,术后28 d处死。处死后收集大鼠主动脉术标本。分别进行下列检测:1血管形态学检测:切片HE染色;2SP染色免疫组织化学检测RGC-32表达,高倍光学显微镜下观察并计算阳性细胞百分比;3Real Time RT-PCR检测各组RGC-32在mRNA水平的表达情况。结果:手术组与正常对比,血管内膜面积增多,差异有统计学意义(P<0.05),手术组与对照组相比,RGC-32明显升高,且RGC-32平滑肌升高更明显,手术组RGC-32阳性表达及RGC-32mRNA水平明显高于对照组(P<0.05)。结论:大鼠主动脉损伤后RGC-32表达水平均明显升高,可为治疗新生内膜增生寻找新的靶点。Objective To investigate the ExpresSion of RGC -32 in the aorta of rats after balloon injury , Ultimately,to create the theoretical foundation for RGC - 32 as a new target treating restenosis after angioplasty. Method The sixteen SD male rat were randomly separated into two groups ： control group（ n = 8 ）, balloon - injured group （ n = 8 ）. The model of rat aorta injury was established with a 2F Fogarty catheter. The vascular tissues were harvested at 28 days after the operation respectively. （1）Histomorphology ： After HE staining and observation using optical microscopy, the intima area of all was measured by computer graph analysis system;（2）Immunohistochemistry （ SP method） was used to determine the differential expressional level of RGC - 32 with time. The slides were observed by microscopy at high magnification at each time period and proportion of positive cells were calculated;（3）The expression level of RGC -32 mRNA were detected with the method of Real Time RT- PCR. Results Intima areamedia area ratio（IA/MA） was found increasing in alioon- injured group （vs control, P 〈 0.05 ）. The amount of RGC - 32 positive cells and the expression of RGC - 32 mRNA increased than control group, the difference was statistically significant （ P 〈 0.05 ）. Conclusion The expression of RGC - 32 increased in Rat Injured Aorta, RGC - 32 may emerge as a promising target of therapy for restenosis.

关 键 词：闭塞性动脉硬化 再狭窄 血管平滑肌 RGC-32 

分 类 号：R654.3[医药卫生—外科学]