hUPF1的两个功能位点对调控ZNF268基因转录活性的影响  

The Effect on Transcriptional Activity of ZNF268 Gene by the Two Functional Sites of hUPF1

作　　者：朱晨刚[1,2] 郭明雄[2] 邵焕杰[3] 

机构地区：[1]贵州师范学院化学与生命科学学院,贵州贵阳550018 [2]武汉大学生命科学学院,湖北武汉430072 [3]陕西师范大学生命科学学院,陕西西安710119

出　　处：《安徽师范大学学报（自然科学版）》2016年第1期60-64,共5页Journal of Anhui Normal University(Natural Science)

基　　金：国家自然科学基金(30500266;31271511);贵州师范学院自然科学研究基金(12BS025)

摘　　要：h UPF1是人类无义介导mRNA降解途径的关键因子,前期研究结果显示h UPF1能够调控ZNF268基因的转录活性.为研究h UPF1的两个功能位点对调控ZNF 268转录活性的影响,使用双荧光报告实验体系,在He La细胞中转染R1R-DE637AA、R1R-RR857AA或质粒组合,然后测量ZNF 268启动子报告质粒p GL3(-37/1234)和p GL3(589/760)的活性.结果显示h UPF1的任何一个功能位点缺失都导致ZNF 268转录活性下降.为排除内源性h UPF1对实验结果的影响,使用RNA干扰的方法降解内源性h UPF1,再转染R1R-DE637AA或R1R-RR857AA质粒.实验结果得到进一步证实.研究表明h UPF1的两个功能位点对调控ZNF 268基因的启动子活性都是必要的.Human UPF1,a key player of mankind nonsense-mediated mRNA decay,was showed that it could regulate the transcriptional activity of ZNF 268 gene. In order to study the effect of the two functional sites of h UPF1 in this process,HeLa cells were transiently transfected with R1R-DE637 AA,R1R-RR857 AA or mixture of plasmids,and then the activity of ZNF268 promoter reporter plasmids of pGL3（-37 /1234） and p GL3（ 589 /760）were measured by double fluorescent report experiments. Results showed that any lack of a functional site of h UPF1 were resulting in decreased ZNF 268 transcriptional activity. To exclude the possible disturbance by internal hUPF1,which was then eliminated by RNAi,the experiments were repeated. Results were confirmed again. This study demonstrated that the two functional sites of h UPF1 were required for the regulation of transcription of ZNF 268 gene.

关 键 词：无义介导mRNA降解 hUPF1 ZNF 268基因 转录调控 

分 类 号：Q344.14[生物学—遗传学]