TGF-β_1在烟雾暴露大鼠肺血管的表达及其与肺血管重塑的关系  

TGF-β_1 Expression in Pulmonary Vessels of Cigarette Smoke-exposed Rats and Its Relationship with Pulmonary Vascular Remodeling

作　　者：王焕[1] 曾玉兰[1] 彭红星[1] 杨荣时[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院呼吸内科,武汉430077

出　　处：《华中科技大学学报（医学版）》2016年第1期12-15,共4页Acta Medicinae Universitatis Scientiae et Technologiae Huazhong

基　　金：湖北省自然科学基金资助项目(No.2013CFB078)

摘　　要：目的通过制备大鼠长期吸烟模型,观察TGF-β_1在烟雾暴露大鼠肺血管中表达的变化,探讨肺血管重塑形成的原因及其机制。方法将60只健康SD雄性大鼠随机分为对照组、烟雾暴露2、4、8、12周组。通过肺组织的苏木精-伊红染色和α-SMA染色切片观察肺血管重塑的程度;通过免疫组化法观察和检测TGF-β_1蛋白在肺动脉中的相对表达。结果 1各组肺血管壁厚度和血管直径的比值(WT%)分别为(21.17±1.43)%,(31.26±1.71)%,(39.95±1.19)%,(50.54±1.49)%,(61.42±1.67)%;各组完全肌化血管比例分别是(3.33±0.15)%,(6.67±0.54)%,(17.34±1.49)%,(27.37±1.49)%,(35.45±1.83)%,烟雾暴露组WT%和完全肌化血管比例均较对照组明显增大,且各组之间差异均有统计学意义(均P<0.05)。2各组TGF-β_1蛋白表达结果依次为(0.041±0.012),(0.326±0.019),(0.343±0.012),(0.448±0.017),(0.480±0.015),各组之间差异有统计学意义(P<0.05)。3TGF-β_1蛋白表达与WT%和完全肌化血管比例呈显著正相关(r值分别为0.902、0.844,均P<0.01)。结论肺血管TGF-β_1表达上调可能参与烟雾暴露大鼠肺血管重塑的形成过程,且与烟雾暴露时间相关。Objective To investigate the variations of TGF-β_1expression in pulmonary vessels in rats exposed to cigarette smoke,and the causes and mechanisms of pulmonary vascular remodeling.Methods Sixty SD male rats were randomly divided into control group and smoke exposure groups（2-,4-,8-,12-week groups in terms of different exposure time）.Pulmonary remodeling was evaluated by using HE andα-SMA staining.Immunohistochemistry was preformed to determine the expression of TGF-β_1protein in the pulmonary artery.Results 1The wall thickness（WT）of the pulmonary artery was（31.26±1.71）%,（39.95±1.19）%,（50.54±1.49）%,（61.42±1.67）%in 2-,4-,8-,and 12-week smoke exposure groups,respectively,significantly greater than that in control group[（21.17±1.43）%]（all P〈0.05）.The proportion of muscularized vessels was（6.67±0.54）%,（17.34±1.49）%,（27.37±1.49）%,and（35.45±1.83）%,respectively,in the four smoke exposure groups,significantly higher than that in the control group[（3.33±0.15）%]（all P〈0.05）.2The TGF-β_1protein expression in the pulmonary artery was（0.041±0.012）,（0.326±0.019）,（0.343±0.012）,（0.448±0.017）,and（0.480±0.015）in the five groups,respectively.There was significant difference in the expression of TGF-β_1protein between groups（P〈0.05）.3The expression of TGF-β_1protein was positively correlated with the WT and proportion of muscularized vessels（r=0.902,P〈0.01;r=0.844,P〈0.01）.Conclusion Up-regulation of TGF-β_1expression in pulmonary vessels may participate in the pulmonary vascular remodeling in rats,and the expression of TGF-β_1protein is positively correlated with the exposure time of cigarette smoke.

关 键 词：TGF-Β1 吸烟 肺血管重塑 肺动脉高压 平滑肌 增殖 

分 类 号：R163[医药卫生—公共卫生与预防医学]